ArticleDNA research : an international journal for rapid publication of reports on genes and genomes2012
Alternative splicing regulates targeting of malate dehydrogenase in Yarrowia lipolytica.
Article in DNA research : an international journal for rapid publication of reports on genes and genomes, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 55 citations in OpenAlex.
- The Key Enzymes of Carbon Metabolism and the Glutathione Antioxidant System ProtectJournal of fungi (Basel, Switzerland) · 2024Article
- Transcriptome analysis reveals multiple targets of erythritol-related transcription factor EUF1 in unconventional yeast Yarrowia Lipolytica.Microbial cell factories · 2024Article
- Replicative aging in yeast involves dynamic intron retention patterns associated with mRNA processing/export and protein ubiquitination.Microbial cell (Graz, Austria) · 2024Article
- Diversity, Lifestyle, Genomics, and Their Functional Role ofJournal of fungi (Basel, Switzerland) · 2023Review
- Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots.Antioxidants (Basel, Switzerland) · 2022Article
- Dissecting carbon metabolism ofComputational and structural biotechnology journal · 2022Article
- Yeasts of the Blastobotrys genus are promising platform for lipid-based fuels and oleochemicals production.Applied microbiology and biotechnology · 2021Review
- BiFC Method Based on Intraorganellar Protein Crowding Detects Oleate-Dependent Peroxisomal Targeting ofInternational journal of molecular sciences · 2021Article
- A piggybacking mechanism enables peroxisomal localization of the glyoxylate cycle enzyme Mdh2 in yeast.Journal of cell science · 2020Article
- Microbial Upgrading of Acetate into Value-Added Products-Examining Microbial Diversity, Bioenergetic Constraints and Metabolic Engineering Approaches.International journal of molecular sciences · 2020Review
- Origin, conservation, and loss of alternative splicing events that diversify the proteome in Saccharomycotina budding yeasts.RNA (New York, N.Y.) · 2020Article
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- YaliBricks, a versatile genetic toolkit for streamlined and rapid pathway engineering inMetabolic engineering communications · 2017Article
- A molecular genetic toolbox forBiotechnology for biofuels · 2017Article
- The functional readthrough extension of malate dehydrogenase reveals a modification of the genetic code.Open biology · 2016Article
- Deciphering how LIP2 and POX2 promoters can optimally regulate recombinant protein production in the yeast Yarrowia lipolytica.Microbial cell factories · 2016Article
- Intron retention-dependent gene regulation in Cryptococcus neoformans.Scientific reports · 2016Article
- Role of Pex11p in Lipid Homeostasis in Yarrowia lipolytica.Eukaryotic cell · 2015Article
- The evolution of Jen3 proteins and their role in dicarboxylic acid transport in Yarrowia.MicrobiologyOpen · 2015Article
- Origin of spliceosomal introns and alternative splicing.Cold Spring Harbor perspectives in biology · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alternative pre-mRNA splicing is a major mechanism contributing to the proteome complexity of most eukaryotes, especially mammals. In less complex organisms, such as yeasts, the numbers of genes that contain introns are low and cases of alternative splicing (AS) with functional implications are rare. We report the first case of AS with functional consequences in the yeast Yarrowia lipolytica. The splicing pattern was found to govern the cellular localization of malate dehydrogenase, an enzyme of the central carbon metabolism. This ubiquitous enzyme is involved in the tricarboxylic acid cycle in mitochondria and in the glyoxylate cycle, which takes place in peroxisomes and the cytosol. In Saccharomyces cerevisiae, three genes encode three compartment-specific enzymes. In contrast, only two genes exist in Y. lipolytica. One gene (YlMDH1, YALI0D16753g) encodes a predicted mitochondrial protein, whereas the second gene (YlMDH2, YALI0E14190g) generates the cytosolic and peroxisomal forms through the alternative use of two 3'-splice sites in the second intron. Both splicing variants were detected in cDNA libraries obtained from cells grown under different conditions. Mutants expressing the individual YlMdh2p isoforms tagged with fluorescent proteins confirmed that they localized to either the cytosolic or the peroxisomal compartment.
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