ArticleCurrent genetics2012
Genetic basis of destruxin production in the entomopathogen Metarhizium robertsii.
Article in Current genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Lecanicillium aphanocladii: a biocontrol agent against insect pests and phytopathogens.Pest management science · 2026Review
- The Isolation, Structural Characterization, and Biosynthetic Pathway of Unguisin from the Marine-Derived FungusMarine drugs · 2025Article
- Integrative taxonomy of Metarhizium anisopliae species complex, based on phylogenomics combined with morphometrics, metabolomics, and virulence data.IMA fungus · 2024Article
- Article
- Effects of passages through an insect or a plant on virulence and physiological properties of the fungusPeerJ · 2023Article
- Profiling Destruxin Synthesis by Specialist and Generalist Metarhizium Insect Pathogens during Coculture with Plants.Applied and environmental microbiology · 2022Article
- Genomic and Experimental Analysis of the Insecticidal Factors Secreted by the Entomopathogenic FungusJournal of fungi (Basel, Switzerland) · 2022Article
- Interaction betweenBiology · 2021Article
- Biosynthesis of depsipeptides, or Depsi: The peptides with varied generations.Protein science : a publication of the Protein Society · 2020Review
- Secondary metabolites from hypocrealean entomopathogenic fungi: genomics as a tool to elucidate the encoded parvome.Natural product reports · 2020Review
- Comparative genomics of Alternaria species provides insights into the pathogenic lifestyle of Alternaria brassicae - a pathogen of the Brassicaceae family.BMC genomics · 2019Article
- Non-ribosomal Peptide Synthetase Gene Clusters in the Human Pathogenic FungusFrontiers in microbiology · 2019Article
- Swainsonine Biosynthesis Genes in Diverse Symbiotic and Pathogenic Fungi.G3 (Bethesda, Md.) · 2017Article
- The Insect Pathogens.Microbiology spectrum · 2017Review
- Article
- Genome sequence and comparative analysis of clavicipitaceous insect-pathogenic fungus Aschersonia badia with Metarhizium spp.BMC genomics · 2016Article
- Metabolic Conservation and Diversification ofFrontiers in microbiology · 2016Article
- Production of destruxins from Metarhizium spp. fungi in artificial medium and in endophytically colonized cowpea plants.PloS one · 2014Article
- Metabolomics reveals the heterogeneous secretome of two entomopathogenic fungi to ex vivo cultured insect tissues.PloS one · 2013Article
Corrections and comments
- Erratum issuedSun-Moon, Yong [corrected to Moon, Yong-Sun]
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Destruxins are among the most exhaustively researched secondary metabolites of entomopathogenic fungi, yet definitive evidence for their roles in pathogenicity and virulence has yet to be shown. To establish the genetic bases for the biosynthesis of this family of depsipeptides, we identified a 23,792-bp gene in Metarhizium robertsii ARSEF 2575 containing six complete nonribosomal peptide synthetase modules, with an N-methyltransferase domain in each of the last two modules. This domain arrangement is consistent with the positioning of the adjacent amino acids N-methyl-L: -valine and N-methyl-L: -alanine within the depsipeptide structure of destruxin. DXS expression levels in vitro and in vivo exhibited comparable patterns, beginning at low levels during the early growth phases and increasing with time. Targeted gene knockout using Agrobacterium-mediated transformation produced mutants that failed to synthesize destruxins, in comparison with wild type and ectopic control strains, indicating the involvement of this gene in destruxin biosynthesis. The destruxin synthetase (DXS) disruption mutant was as virulent as the control strain when conidial inoculum was topically applied to larvae of Spodoptera exigua, Galleria mellonella, and Tenebrio molitor indicating that destruxins are dispensable for virulence in these insect hosts. The DXS mutants exhibited no other detectable changes in morphology and development.
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