ArticleNature cell biology2012
Single-molecule assays reveal that RNA localization signals regulate dynein-dynactin copy number on individual transcript cargoes.
Article in Nature cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 83 citations in OpenAlex.
- How energy determines spatial localisation and copy number of molecules in neurons.Nature communications · 2025Article
- Tropomyosin 1-I/C coordinates kinesin-1 and dynein motors during oskar mRNA transport.Nature structural & molecular biology · 2024Article
- Proteins rather than mRNAs regulate nucleation and persistence of Oskar germ granules in Drosophila.Cell reports · 2023Article
- An RNA-based feed-forward mechanism ensures motor switching in oskar mRNA transport.The Journal of cell biology · 2023Article
- Sequence Determinants of TDP-43 Ribonucleoprotein Condensate Formation and Axonal Transport in Neurons.Frontiers in cell and developmental biology · 2022Article
- Optical tweezer and TIRF microscopy for single molecule manipulation of RNA/DNA nanostructures including their rubbery property and single molecule counting.Biophysics reports · 2021Article
- Molecular mechanisms behind mRNA localization in axons.Open biology · 2020Review
- Polysomes identified by live imaging of nascent peptides are stalled in hippocampal and cortical neurites.Learning & memory (Cold Spring Harbor, N.Y.) · 2019Article
- Close encounters of the RNAi kind: the silencing life of the adherens junctions.Current opinion in cell biology · 2018Review
- SpliceRCA:ACS central science · 2018Article
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- Expanding Axonal Transcriptome Brings New Functions for Axonally Synthesized Proteins in Health and Disease.The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry · 2018Review
- Cue-Polarized Transport of β-actin mRNA Depends on 3'UTR and Microtubules in Live Growth Cones.Frontiers in cellular neuroscience · 2018Article
- Intracellular Cargo Transport by Kinesin-3 Motors.Biochemistry. Biokhimiia · 2017Review
- Advancement of the Emerging Field of RNA Nanotechnology.ACS nano · 2017Review
- Loss of function of the Drosophila Ninein-related centrosomal protein Bsg25D causes mitotic defects and impairs embryonic development.Biology open · 2016Article
- Cotranslational association of mRNA encoding subunits of heteromeric ion channels.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Community effects in regulation of translation.eLife · 2016Article
- Independent and coordinate trafficking of single Drosophila germ plasm mRNAs.Nature cell biology · 2015Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Subcellular localization of mRNAs by cytoskeletal motors plays critical roles in the spatial control of protein function. However, optical limitations of studying mRNA transport in vivo mean that there is little mechanistic insight into how transcripts are packaged and linked to motors, and how the movement of mRNA-motor complexes on the cytoskeleton is orchestrated. Here, we have reconstituted transport of mRNPs containing specific RNAs in vitro. We show directly that mRNAs that are either apically localized or non-localized in Drosophila melanogaster embryos associate with the dynein motor and move bidirectionally on individual microtubules, with localizing mRNPs exhibiting a strong minus-end-directed bias. Single-molecule fluorescence measurements reveal that RNA localization signals increase the average number of dynein and dynactin components recruited to individual mRNPs. We find that, surprisingly, individual RNA molecules are present in motile mRNPs in vitro and provide evidence that this is also the case in vivo. Thus, RNA oligomerization is not obligatory for transport. Our findings lead to a model in which RNA localization signals produce highly polarized distributions of transcript populations through modest changes in motor copy number on single mRNA molecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.