ArticleEukaryotic cell2012
Role of ATG8 and autophagy in programmed nuclear degradation in Tetrahymena thermophila.
Article in Eukaryotic cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 2 of them syntheses that pooled it.
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Who cites it
33 citing papers in PubMed, 2 syntheses or guidelines pooled it, 59 citations in OpenAlex.
- Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)Autophagy · 2021Guideline
- Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).Autophagy · 2016Guideline
- Autophagy and the Mitochondrial Lon1 Protease Are Necessary for Botrytis cinerea Heat Adaptation.Molecular microbiology · 2025Article
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- Close cooperation between Semi1 and Semi2 proteins is essential for pronuclear positioning inMolecular biology of the cell · 2025Article
- The function of the ATG8 in the cilia and cortical microtubule maintenance of Euplotes amieti.Protoplasma · 2024Article
- Dynamic Localization of Endoplasmic Reticulum during Tetrahymena Conjugation.microPublication biology · 2024Article
- Autophagy in protists and their hosts: When, how and why?Autophagy reports · 2023Article
- Snf2 Proteins Are Required to Generate Gamete Pronuclei inMicroorganisms · 2022Article
- Evolutionary diversification of the autophagy-related ubiquitin-like conjugation systems.Autophagy · 2022Article
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- The necessity of nucleophagic modality.Autophagy · 2022Article
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- The histone chaperone Nrp1 is required for chromatin stability and nuclear division in Tetrahymena thermophila.Epigenetics & chromatin · 2021Article
- Autophagy of the Nucleus in Health and Disease.Frontiers in cell and developmental biology · 2021Review
- The opposing roles of the mTOR signaling pathway in different phases of human umbilical cord blood-derived CD34Stem cells (Dayton, Ohio) · 2020Article
- Comprehensive Chromosome End Remodeling during Programmed DNA Elimination.Current biology : CB · 2020Article
- Diversification of CORVET tethers facilitates transport complexity inJournal of cell science · 2020Article
- The Transmembrane Protein Semi1 Positions Gamete Nuclei for Reciprocal Fertilization in Tetrahymena.iScience · 2020Article
- Symbiotic Origin of Apoptosis.Results and problems in cell differentiation · 2020Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autophagy is an evolutionarily conserved mechanism for the degradation of cellular components, but its role in enucleation during differentiation has not been established. Tetrahymena thermophila is a unicellular eukaryote with two functionally distinct nuclei, the somatic (macro-) and the germ line (micro-) nuclei. These nuclei are produced during sexual reproduction (conjugation), which involves differentiation and selective degradation of several specific nuclei. To examine the role of autophagy in nuclear degradation, we studied the function of two ATG8 genes in Tetrahymena. Through fluorescent protein tagging, we found that both proteins are targeted to degrading nuclei at specific stages, with some enrichment on the nuclear periphery, suggesting the formation of autophagosomes surrounding these nuclei. In addition, ATG8 knockout mutant cells showed a pronounced delay in nuclear degradation without apparently preventing the completion of other developmental events. This evidence provided direct support for a critical role for autophagy in programmed nuclear degradation. The results also showed differential roles for two ATG8 genes, with ATG8-65 playing a more significant role in starvation than ATG8-2, although both are important in nuclear degradation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.