ArticleChemistry & biology2012
In vivo characterization of nonribosomal peptide synthetases NocA and NocB in the biosynthesis of nocardicin A.
Article in Chemistry & biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Dehydroamino acid residues in bioactive natural products.Natural product reports · 2024Review
- Structural advances toward understanding the catalytic activity and conformational dynamics of modular nonribosomal peptide synthetases.Natural product reports · 2023Review
- Structural Studies of Modular Nonribosomal Peptide Synthetases.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Site-Directed Mutagenesis of Large Biosynthetic Gene ClustersACS synthetic biology · 2020Article
- Pass-back chain extension expands multimodular assembly line biosynthesis.Nature chemical biology · 2020Article
- Bioactive molecules from Nocardia: diversity, bioactivities and biosynthesis.Journal of industrial microbiology & biotechnology · 2019Review
- Mechanism of Integrated β-Lactam Formation by a Nonribosomal Peptide Synthetase during Antibiotic Synthesis.Biochemistry · 2018Article
- Heteroatom-Heteroatom Bond Formation in Natural Product Biosynthesis.Chemical reviews · 2017Review
- Convergent biosynthetic pathways to β-lactam antibiotics.Current opinion in chemical biology · 2016Review
- β-Lactam formation by a non-ribosomal peptide synthetase during antibiotic biosynthesis.Nature · 2015Article
- Epimerization and substrate gating by a TE domain in β-lactam antibiotic biosynthesis.Nature chemical biology · 2014Article
- Stereocontrolled syntheses of peptide thioesters containing modified seryl residues as probes of antibiotic biosynthesis.The Journal of organic chemistry · 2013Article
- Non-ribosomal propeptide precursor in nocardicin A biosynthesis predicted from adenylation domain specificity dependent on the MbtH family protein NocI.Journal of the American Chemical Society · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Two nonribosomal peptide synthetases (NRPS), NocA and NocB, together comprising five modules, are essential for the biosynthesis of the D,L,D configured tripeptide backbone of the monocyclic β-lactam nocardicin A. We report a double replacement gene strategy in which point mutations were engineered in the two encoding NRPS genes without disruption of the nocABC operon by placing selective markers in adjacent genes. A series of mutants was constructed to inactivate the thiolation (T) domain of each module and to evaluate an HHxxxDR catalytic motif in NocA and an atypical extended histidine motif in NocB. The loss of nocardicin A production in each of the T domain mutants indicates that all five modules are essential for its biosynthesis. Conversely, production of nocardicin A was not affected by mutation of the NocB histidine motif or the R828G mutation in NocA.
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