ArticleVirology2012
MuLV IN mutants responsive to HDAC inhibitors enhance transcription from unintegrated retroviral DNA.
Article in Virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Brief Histories of Retroviral Integration Research and Associated International Conferences.Viruses · 2024Review
- HIV Preintegration Transcription and Host Antagonism.Current HIV research · 2023Article
- Two lymphoid cell lines potently silence unintegrated HIV-1 DNAs.Retrovirology · 2022Article
- Silencing of Unintegrated Retroviral DNAs.Viruses · 2021Review
- Distinct Antiretroviral Mechanisms Elicited by a Viral Mutagen.Journal of molecular biology · 2021Article
- Mutations altering acetylated residues in the CTD of HIV-1 integrase cause defects in proviral transcription at early times after integration of viral DNA.PLoS pathogens · 2020Article
- Going beyond Integration: The Emerging Role of HIV-1 Integrase in Virion Morphogenesis.Viruses · 2020Review
- Disrupting MLV integrase:BET protein interaction biases integration into quiescent chromatin and delays but does not eliminate tumor activation in a MYC/Runx2 mouse model.PLoS pathogens · 2019Article
- Unintegrated HIV-1 DNAs are loaded with core and linker histones and transcriptionally silenced.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Identification of Extrachromosomal Linear microDNAs Interacted with microRNAs in the Cell Nuclei.Cells · 2019Article
- Repression of the Chromatin-Tethering Domain of Murine Leukemia Virus p12.Journal of virology · 2016Article
- Histones Are Rapidly Loaded onto Unintegrated Retroviral DNAs Soon after Nuclear Entry.Cell host & microbe · 2016Article
- Poly(ADP-ribose) polymerase-1 silences retroviruses independently of viral DNA integration or heterochromatin formation.The Journal of general virology · 2016Article
- HIV-1 latency and virus production from unintegrated genomes following direct infection of resting CD4 T cells.Retrovirology · 2016Article
- Structural and sequencing analysis of local target DNA recognition by MLV integrase.Nucleic acids research · 2015Article
- Improved retroviral episome transfer of transcription factors enables sustained cell fate modification.Gene therapy · 2014Article
- MLV based viral-like-particles for delivery of toxic proteins and nuclear transcription factors.Biomaterials · 2014Article
- Altering murine leukemia virus integration through disruption of the integrase and BET protein family interaction.Nucleic acids research · 2014Article
- Molecular mechanisms of retroviral integration site selection.Nucleic acids research · 2014Article
- Development of an enzyme-linked immunosorbent assay based on the murine leukemia virus p30 capsid protein.Journal of virological methods · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
For Moloney murine leukemia virus (M-MuLV), sustained viral infections require expression from an integrated provirus. For many applications, non-integrating retroviral vectors have been utilized to avoid the unwanted effects of integration, however, the level of expression from unintegrated DNA is significantly less than that of integrated provirus. We find that unintegrated DNA expression can be increased in the presence of HDAC inhibitors, such as TSA, when applied in combination with integrase (IN) mutations. These mutants include an active site mutation as well as catalytically active INs bearing mutations of K376 in the MuLV C-terminal domain of IN. MuLV IN K376 is homologous to K266 in HIV-1 IN, a known substrate for acetylation. The MuLV IN protein is acetylated by p300 in vitro, however, the effect of HDAC inhibitors on gene expression from unintegrated DNA is not dependent on the acetylation state of MuLV IN K376.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.