ArticleBiotechnology for biofuels2012
Functional expression and characterization of five wax ester synthases in Saccharomyces cerevisiae and their utility for biodiesel production.
Article in Biotechnology for biofuels, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 141 citations in OpenAlex.
- Engineering yeast for tailored fatty acid profiles.Applied microbiology and biotechnology · 2025Review
- Advanced metabolic Engineering strategies for the sustainable production of free fatty acids and their derivatives using yeast.Journal of biological engineering · 2024Review
- Saccharomyces cerevisiae cellular engineering for the production of FAME biodiesel.AMB Express · 2024Article
- Exploring aRSC chemical biology · 2023Article
- Alcohol acyltransferases for the biosynthesis of esters.Biotechnology for biofuels and bioproducts · 2023Review
- Directed evolution of a wax ester synthase for production of fatty acid ethyl esters in Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2023Article
- The production of wax esters in transgenic plants: towards a sustainable source of bio-lubricants.Journal of experimental botany · 2022Review
- Escherichia coli coculture for de novo production of esters derived of methyl-branched alcohols and multi-methyl branched fatty acids.Microbial cell factories · 2022Article
- Metabolic engineering Yarrowia lipolytica for a dual biocatalytic system to produce fatty acid ethyl esters from renewable feedstock in situ and in one pot.Applied microbiology and biotechnology · 2021Article
- Engineering oleaginous yeast Rhodotorula toruloides for overproduction of fatty acid ethyl esters.Biotechnology for biofuels · 2021Article
- The Studies in Constructing Yeast Cell Factories for the Production of Fatty Acid Alkyl Esters.Frontiers in bioengineering and biotechnology · 2021Review
- Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica.MicrobiologyOpen · 2020Article
- Cellular and metabolic engineering of oleaginous yeastEngineering in life sciences · 2019Review
- Engineering Saccharomyces cerevisiae cells for production of fatty acid-derived biofuels and chemicals.Open biology · 2019Review
- Microbial biosynthesis of lactate esters.Biotechnology for biofuels · 2019Article
- EngineeringPeerJ · 2019Article
- Structural and Biochemical Studies of a Biocatalyst for the Enzymatic Production of Wax Esters.ACS catalysis · 2018Article
- Characterization and engineering of a dual-function diacylglycerol acyltransferase in the oleaginous marine diatomBiotechnology for biofuels · 2018Article
- Biosynthesis and engineering of kaempferol in Saccharomyces cerevisiae.Microbial cell factories · 2017Article
- EngineeringACS omega · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWax ester synthases (WSs) can synthesize wax esters from alcohols and fatty acyl coenzyme A thioesters. The knowledge of the preferred substrates for each WS allows the use of yeast cells for the production of wax esters that are high-value materials and can be used in a variety of industrial applications. The products of WSs include fatty acid ethyl esters, which can be directly used as biodiesel.
resultsHere, heterologous WSs derived from five different organisms were successfully expressed and evaluated for their substrate preference in Saccharomyces cerevisiae. We investigated the potential of the different WSs for biodiesel (that is, fatty acid ethyl esters) production in S. cerevisiae. All investigated WSs, from Acinetobacter baylyi ADP1, Marinobacter hydrocarbonoclasticus DSM 8798, Rhodococcus opacus PD630, Mus musculus C57BL/6 and Psychrobacter arcticus 273-4, have different substrate specificities, but they can all lead to the formation of biodiesel. The best biodiesel producing strain was found to be the one expressing WS from M. hydrocarbonoclasticus DSM 8798 that resulted in a biodiesel titer of 6.3 mg/L. To further enhance biodiesel production, acetyl coenzyme A carboxylase was up-regulated, which resulted in a 30% increase in biodiesel production.
conclusionsFive WSs from different species were functionally expressed and their substrate preference characterized in S. cerevisiae, thus constructing cell factories for the production of specific kinds of wax ester. WS from M. hydrocarbonoclasticus showed the highest preference for ethanol compared to the other WSs, and could permit the engineered S. cerevisiae to produce biodiesel.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.