ArticlePloS one2012
Insulin-like growth factors promote vasculogenesis in embryonic stem cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 74 citations in OpenAlex.
- Promising Preventive Strategies for Intraventricular Hemorrhage in Preterm Neonates: A Critical Review.Journal of clinical medicine · 2025Review
- Insulin-Like Growth Factors, Binding Proteins and Their Role in Pregnancy in Patients With Diabetes.Journal of diabetes research · 2025Review
- Evaluation of recombinant human IGF-1/IGFBP-3 on intraventricular hemorrhage prevention and survival in the preterm rabbit pup model.Scientific reports · 2023Article
- Effect of insulin-like growth factor system on luteinising angiogenesis.Reproduction & fertility · 2023Article
- Indirect Immobilised Jagged-1 Enhances Matrisome Proteins Associated with Osteogenic Differentiation of Human Dental Pulp Stem Cells: A Proteomic Study.International journal of molecular sciences · 2022Article
- Review
- Macrophage-derived IGF-1 protects the neonatal intestine against necrotizing enterocolitis by promoting microvascular development.Communications biology · 2022Article
- Insulin-like growth factor system components expressed at the conceptus-maternal interface during the establishment of equine pregnancy.Frontiers in veterinary science · 2022Article
- Article
- Essential role of theLife science alliance · 2020Article
- Insulin receptor-mediated signaling regulates pluripotency markers and lineage differentiation.Molecular metabolism · 2018Article
- Article
- Up-regulated basigin-2 in microglia induced by hypoxia promotes retinal angiogenesis.Journal of cellular and molecular medicine · 2017Article
- Retinopathy of prematurity: inflammation, choroidal degeneration, and novel promising therapeutic strategies.Journal of neuroinflammation · 2017Review
- Type I insulin-like growth factor receptor signaling in hematological malignancies.Oncotarget · 2017Review
- Regulation of Osteogenic Differentiation of Placental-Derived Mesenchymal Stem Cells by Insulin-Like Growth Factors and Low Oxygen Tension.Stem cells international · 2017Article
- β-cell insulin receptor deficiency during in utero development induces an islet compensatory overgrowth response.Oncotarget · 2016Article
- Mesenchymal stem cells-seeded bio-ceramic construct for bone regeneration in large critical-size bone defect in rabbit.Journal of stem cells & regenerative medicine · 2016Article
- IGF-I Signaling in Osterix-Expressing Cells Regulates Secondary Ossification Center Formation, Growth Plate Maturation, and Metaphyseal Formation During Postnatal Bone Development.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2015Article
- Chemically-defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells.Stem cell research · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
The ability of embryonic stem cells to differentiate into endothelium and form functional blood vessels has been well established and can potentially be harnessed for therapeutic angiogenesis. However, after almost two decades of investigation in this field, limited knowledge exists for directing endothelial differentiation. A better understanding of the cellular mechanisms regulating vasculogenesis is required for the development of embryonic stem cell-based models and therapies. In this study, we elucidated the mechanistic role of insulin-like growth factors (IGF1 and 2) and IGF receptors (IGFR1 and 2) in endothelial differentiation using an embryonic stem cell embryoid body model. Both IGF1 or IGF2 predisposed embryonic stem to differentiate towards a mesodermal lineage, the endothelial precursor germ layer, as well as increased the generation of significantly more endothelial cells at later stages. Inhibition of IGFR1 signaling using neutralizing antibody or a pharmacological inhibitor, picropodophyllin, significantly reduced IGF-induced mesoderm and endothelial precursor cell formation. We confirmed that IGF-IGFR1 signaling stabilizes HIF1α and leads to up-regulation of VEGF during vasculogenesis in embryoid bodies. Understanding the mechanisms that are critical for vasculogenesis in various models will bring us one step closer to enabling cell based therapies for neovascularization.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.