ArticlePloS one2012
Identification of mammalian protein quality control factors by high-throughput cellular imaging.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Hypoxia and the Kynurenine Pathway: Implications and Therapeutic Prospects in Alzheimer's Disease.Oxidative medicine and cellular longevity · 2021Review
- eIF-Three to Tango: emerging functions of translation initiation factor eIF3 in protein synthesis and disease.Journal of molecular cell biology · 2020Review
- Reporter-Based Screens for the Ubiquitin/Proteasome System.Frontiers in chemistry · 2020Review
- High-Throughput Imaging for the Discovery of Cellular Mechanisms of Disease.Trends in genetics : TIG · 2017Review
- Article
- A high-content imaging-based screening pipeline for the systematic identification of anti-progeroid compounds.Methods (San Diego, Calif.) · 2016Article
- Deep Imaging: the next frontier in microscopy.Histochemistry and cell biology · 2014Review
- Review
- Human cytomegalovirus UL76 elicits novel aggresome formation via interaction with S5a of the ubiquitin proteasome system.Journal of virology · 2013Article
- Neurodegenerative Diseases: Multifactorial Conformational Diseases and Their Therapeutic Interventions.Journal of neurodegenerative diseases · 2013Review
- HUWE1 and TRIP12 collaborate in degradation of ubiquitin-fusion proteins and misframed ubiquitin.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Protein Quality Control (PQC) pathways are essential to maintain the equilibrium between protein folding and the clearance of misfolded proteins. In order to discover novel human PQC factors, we developed a high-content, high-throughput cell-based assay to assess PQC activity. The assay is based on a fluorescently tagged, temperature sensitive PQC substrate and measures its degradation relative to a temperature insensitive internal control. In a targeted screen of 1591 siRNA genes involved in the Ubiquitin-Proteasome System (UPS) we identified 25 of the 33 genes encoding for 26S proteasome subunits and discovered several novel PQC factors. An unbiased genome-wide siRNA screen revealed the protein translation machinery, and in particular the EIF3 translation initiation complex, as a novel key modulator of misfolded protein stability. These results represent a comprehensive unbiased survey of human PQC components and establish an experimental tool for the discovery of genes that are required for the degradation of misfolded proteins under conditions of proteotoxic stress.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.