ArticlePloS one2012
RHGF-2 is an essential Rho-1 specific RhoGEF that binds to the multi-PDZ domain scaffold protein MPZ-1 in Caenorhabditis elegans.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- PLEKHG2 and the PLEKHG family: Linking Rho family GTPases to neural development and disorders.Histology and histopathology · 2025Review
- A sequencing-based screening method identifies regulators of EGFR signaling from nonviable mutants inScience signaling · 2025Article
- Article
- Identification and characterization of Crumbs polarity complex proteins in Caenorhabditis elegans.The Journal of biological chemistry · 2022Article
- A familiar study on self-limited childhood epilepsy patients using hIPSC-derived neurons shows a bias towards immaturity at the morphological, electrophysiological and gene expression levels.Stem cell research & therapy · 2021Article
- A genetic screen for temperature-sensitive morphogenesis-defective Caenorhabditis elegans mutants.G3 (Bethesda, Md.) · 2021Article
- Game of Tissues: How the Epidermis ThronesJournal of developmental biology · 2020Review
- RHO-1 and the Rho GEF RHGF-1 interact with UNC-6/Netrin signaling to regulate growth cone protrusion and microtubule organization in Caenorhabditis elegans.PLoS genetics · 2019Article
- The RhoGEF protein Plekhg5 regulates apical constriction of bottle cells during gastrulation.Development (Cambridge, England) · 2018Article
- Tissue-Specific Functions ofG3 (Bethesda, Md.) · 2018Article
- Spatial control of active CDC-42 during collective migration of hypodermal cells in Caenorhabditis elegans.Journal of molecular cell biology · 2016Article
- Identification of Wnt Pathway Target Genes Regulating the Division and Differentiation of Larval Seam Cells and Vulval Precursor Cells in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2015Article
- RHGF-1/PDZ-RhoGEF and retrograde DLK-1 signaling drive neuronal remodeling on microtubule disassembly.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- pix-1 controls early elongation in parallel with mel-11 and let-502 in Caenorhabditis elegans.PloS one · 2014Article
- NG2 regulates directional migration of oligodendrocyte precursor cells via Rho GTPases and polarity complex proteins.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Mechanisms of CDC-42 activation during contact-induced cell polarization.Journal of cell science · 2013Article
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6 authors.
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Abstract
RhoGEF proteins activate the Rho family of small GTPases and thus play a key role in regulating fundamental cellular processes such as cell morphology and polarity, cell cycle progression and gene transcription. We identified a Caenorhabditis elegans RhoGEF protein, RHGF-2, as a binding partner of the C. elegans multi-PDZ domain scaffold protein MPZ-1 (MUPP1 in mammals). RHGF-2 exhibits significant identity to the mammalian RhoGEFs PLEKHG5/Tech/Syx and contains a class I C-terminal PDZ binding motif (SDV) that interacts most strongly to MPZ-1 PDZ domain eight. RHGF-2 RhoGEF activity is specific to the C. elegans RhoA homolog RHO-1 as determined by direct binding, GDP/GTP exchange and serum response element-driven reporter activity. rhgf-2 is an essential gene since rhgf-2 deletion mutants do not elongate during embryogenesis and hatch as short immobile animals that arrest development. Interestingly, the expression of a functional rhgf-2::gfp transgene appears to be exclusively neuronal and rhgf-2 overexpression results in loopy movement with exaggerated body bends. Transient expression of RHGF-2 in N1E-115 neuroblastoma cells prevents neurite outgrowth similar to constitutive RhoA activation in these cells. Together, these observations indicate neuronally expressed RHGF-2 is an essential RHO-1 specific RhoGEF that binds most strongly to MPZ-1 PDZ domain eight and is required for wild-type C. elegans morphology and growth.
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