ArticlePloS one2012
Highly sensitive detection of Staphylococcus aureus directly from patient blood.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Effectiveness of Practices To Increase Timeliness of Providing Targeted Therapy for Inpatients with Bloodstream Infections: a Laboratory Medicine Best Practices Systematic Review and Meta-analysis.Clinical microbiology reviews · 2016Pooled it
- Bacteriostatic Mechanism of the Ethyl Acetate Extract from the Root ofVeterinary sciences · 2026Article
- Deciphering Bacterial Community Succession and Pathogen Dynamics in ICU Ventilator Circuits Through Full-Length 16S rRNA Sequencing for Mitigating the Risk of Nosocomial Infections.Microorganisms · 2025Article
- Development of a Multiplex Polymerase Chain Reaction-Based DNA Lateral Flow Assay as a Point-of-Care Diagnostic for Fast and Simultaneous Detection of MRSA and Vancomycin Resistance in Bacteremia.Diagnostics (Basel, Switzerland) · 2022Article
- An advanced and efficient asymmetric PCR method for microarray applications.Frontiers in bioengineering and biotechnology · 2022Article
- A 2020 review on the role of procalcitonin in different clinical settings: an update conducted with the tools of the Evidence Based Laboratory Medicine.Annals of translational medicine · 2020Review
- Phage-Mediated Molecular Detection (PMMD): A Novel Rapid Method for Phage-Specific Bacterial Detection.Viruses · 2020Article
- Advances in Directly Amplifying Nucleic Acids from Complex Samples.Biosensors · 2019Review
- Multiplex Detection of Three Select Agents Directly from Blood by Use of the GeneXpert System.Journal of clinical microbiology · 2019Article
- Peptidoglycan binding protein (PGBP)-modified magnetic nanobeads for efficient magnetic capturing of Staphylococcus aureus associated with sepsis in blood.Scientific reports · 2019Article
- A 3D-Printed Millifluidic Platform Enabling Bacterial Preconcentration and DNA Purification for Molecular Detection of Pathogens in Blood.Micromachines · 2018Article
- A rapid colorimetric immunoassay for the detection of pathogenic bacteria on poultry processing plants using cotton swabs and nanobeads.Mikrochimica acta · 2018Article
- Development of An Impedimetric Aptasensor for the Detection of Staphylococcus aureus.International journal of molecular sciences · 2017Article
- Rapid Detection of Bacillus anthracis Bloodstream Infections by Use of a Novel Assay in the GeneXpert System.Journal of clinical microbiology · 2017Article
- Sensitive Detection of Francisella tularensis Directly from Whole Blood by Use of the GeneXpert System.Journal of clinical microbiology · 2017Article
- PCR-based Approaches for the Detection of Clinical Methicillin-resistant Staphylococcus aureus.The open microbiology journal · 2016Article
- Innovative qPCR using interfacial effects to enable low threshold cycle detection and inhibition relief.Science advances · 2015Article
- Resistant Strains of Enterotoxigenic Staphylococcus aureus; Unknown Risk for Multiple Sclerosis Exacerbation.Iranian Red Crescent medical journal · 2015Article
- A Diagnostic Serum Antibody Test for Patients With Staphylococcus aureus Osteomyelitis.Clinical orthopaedics and related research · 2015Article
- Meticillin-resistant Staphylococcus aureus isolated from Iranian hospitals: virulence factors and antibiotic resistance properties.Bosnian journal of basic medical sciences · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundRapid detection of bloodstream infections (BSIs) can be lifesaving. We investigated the sample processing and assay parameters necessary for highly-sensitive detection of bloodstream bacteria, using Staphylococcus aureus as a model pathogen and an automated fluidic sample processing-polymerase chain reaction (PCR) platform as a model diagnostic system. METHODOLOGY/PRINCIPAL
findingsWe compared a short 128 bp amplicon hemi-nested PCR and a relatively shorter 79 bp amplicon nested PCR targeting the S. aureus nuc and sodA genes, respectively. The sodA nested assay showed an enhanced limit of detection (LOD) of 5 genomic copies per reaction or 10 colony forming units (CFU) per ml blood over 50 copies per reaction or 50 CFU/ml for the nuc assay. To establish optimal extraction protocols, we investigated the relative abundance of the bacteria in different components of the blood (white blood cells (WBCs), plasma or whole blood), using the above assays. The blood samples were obtained from the patients who were culture positive for S. aureus. Whole blood resulted in maximum PCR positives with sodA assay (90% positive) as opposed to cell-associated bacteria (in WBCs) (71% samples positive) or free bacterial DNA in plasma (62.5% samples positive). Both the assays were further tested for direct detection of S. aureus in patient whole blood samples that were contemporaneous culture positive. S. aureus was detected in 40/45 of culture-positive patients (sensitivity 89%, 95% CI 0.75-0.96) and 0/59 negative controls with the sodA assay (specificity 100%, 95% CI 0.92-1).
conclusionsWe have demonstrated a highly sensitive two-hour assay for detection of sepsis causing bacteria like S. aureus directly in 1 ml of whole blood, without the need for blood culture.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.