ArticlePloS one2012
Multiple sources of contamination in samples from patients reported to have XMRV infection.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Can Oncogenic Animal Viruses Pose a Threat to Humans?Pathogens (Basel, Switzerland) · 2025Review
- Diversity of RNA viruses in agricultural insects.Computational and structural biotechnology journal · 2023Review
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- Clinical Metagenomic Sequencing for Species Identification and Antimicrobial Resistance Prediction in Orthopedic Device Infection.Journal of clinical microbiology · 2022Article
- Article
- Fake Science: XMRV, COVID-19, and the Toxic Legacy of Dr. Judy Mikovits.AIDS research and human retroviruses · 2020Article
- Laboratory contamination over time during low-biomass sample analysis.Molecular ecology resources · 2019Article
- Chronic viral infections in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS).Journal of translational medicine · 2018Review
- Real-time analysis of nanopore-based metagenomic sequencing from infected orthopaedic devices.BMC genomics · 2018Article
- Molecular Diagnosis of Orthopedic-Device-Related Infection Directly from Sonication Fluid by Metagenomic Sequencing.Journal of clinical microbiology · 2017Article
- The blood DNA virome in 8,000 humans.PLoS pathogens · 2017Article
- Inherent bacterial DNA contamination of extraction and sequencing reagents may affect interpretation of microbiota in low bacterial biomass samples.Gut pathogens · 2016Article
- Endogenous murine leukemia retroviral variation across wild European and inbred strains of house mouse.BMC genomics · 2015Article
- Frequent proviral integration of the human betaretrovirus in biliary epithelium of patients with autoimmune and idiopathic liver disease.Alimentary pharmacology & therapeutics · 2015Article
- Reagent and laboratory contamination can critically impact sequence-based microbiome analyses.BMC biology · 2014Article
- Article
- Conference highlights of the 16th International Conference on Human Retrovirology: HTLV and related retroviruses, 26-30 June 2013, Montreal, Canada.Retrovirology · 2014Article
- Partial molecular cloning of the JHK retrovirus using gammaretrovirus consensus PCR primers.Future virology · 2013Article
- Emerging infectious agents and the nation's blood supply: responding to potential threats in the 21st century.Transfusion · 2013Article
- No evidence of xenotropic murine leukemia virus-related virus transmission by blood transfusion from infected rhesus macaques.Journal of virology · 2013Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 1 country.
Funding
Abstract
Xenotropic murine leukemia virus (MLV)-related retrovirus (XMRV) was reported to be associated with prostate cancer by Urisman, et al. in 2006 and chronic fatigue syndrome (CFS) by Lombardi, et al. in 2009. To investigate this association, we independently evaluated plasma samples from 4 patients with CFS reported by Lombardi, et al. to have XMRV infection and from 5 healthy controls reported to be XMRV uninfected. We also analyzed viral sequences obtained from supernatants of cell cultures found to contain XMRV after coculture with 9 clinical samples from 8 patients. A qPCR assay capable of distinguishing XMRV from endogenous MLVs showed that the viral sequences detected in the CFS patient plasma behaved like endogenous MLVs and not XMRV. Single-genome sequences (N = 89) from CFS patient plasma were indistinguishable from endogenous MLVs found in the mouse genome that are distinct from XMRV. By contrast, XMRV sequences were detected by qPCR in 2 of the 5 plasma samples from healthy controls (sequencing of the qPCR product confirmed XMRV not MLV). Single-genome sequences (N = 234) from the 9 culture supernatants reportedly positive for XMRV were indistinguishable from XMRV sequences obtained from 22Rv1 and XMRV-contaminated 293T cell-lines. These results indicate that MLV DNA detected in the plasma samples from CFS patients evaluated in this study was from contaminating mouse genomic DNA and that XMRV detected in plasma samples from healthy controls and in cultures of patient samples was due to cross-contamination with XMRV (virus or nucleic acid).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.