ArticlePloS one2012
S. pombe kinesins-8 promote both nucleation and catastrophe of microtubules.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 51 citations in OpenAlex.
- Evidence of kinesin motors involved in stable kinetochore assembly during early meiosis.Molecular biology of the cell · 2023Article
- Reliable and robust control of nucleus centering is contingent on nonequilibrium force patterns.iScience · 2023Article
- A Functional Yeast-Based Screen Identifies the Host Microtubule Cytoskeleton as a Target of NumerousInternational journal of molecular sciences · 2023Article
- Mechanochemical tuning of a kinesin motor essential for malaria parasite transmission.Nature communications · 2022Article
- Loss of kinesin-8 improves the robustness of the self-assembled spindle in Schizosaccharomyces pombe.Journal of cell science · 2021Article
- Microtubule-associated proteins and motors required for ectopic microtubule array formation in Saccharomyces cerevisiae.Genetics · 2021Article
- Review
- Bioenergetics of theBiomolecules · 2020Article
- Kinesin-8 and Dis1/TOG collaborate to limit spindle elongation from prophase to anaphase A for proper chromosome segregation in fission yeast.Journal of cell science · 2019Article
- Article
- Emerging Insights into the Function of Kinesin-8 Proteins in Microtubule Length Regulation.Biomolecules · 2018Review
- Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability.Molecular biology of the cell · 2018Article
- Structural basis of human kinesin-8 function and inhibition.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Inositol Pyrophosphate Kinase Asp1 Modulates Chromosome Segregation Fidelity and Spindle Function in Schizosaccharomyces pombe.Molecular and cellular biology · 2016Article
- Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast.Molecular biology of the cell · 2016Article
- α-Synuclein is a Novel Microtubule Dynamase.Scientific reports · 2016Article
- Alp7/TACC-Alp14/TOG generates long-lived, fast-growing MTs by an unconventional mechanism.Scientific reports · 2016Article
- A stable microtubule array drives fission yeast polarity reestablishment upon quiescence exit.The Journal of cell biology · 2015Article
- Mitosis, microtubule dynamics and the evolution of kinesins.Experimental cell research · 2015Review
- Alp7/TACC recruits kinesin-8-PP1 to the Ndc80 kinetochore protein for timely mitotic progression and chromosome movement.Journal of cell science · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The kinesins-8 were originally thought to be microtubule depolymerases, but are now emerging as more versatile catalysts of microtubule dynamics. We show here that S. pombe Klp5-436 and Klp6-440 are non-processive plus-end-directed motors whose in vitro velocities on S. pombe microtubules at 7 and 23 nm s(-1) are too slow to keep pace with the growing tips of dynamic interphase microtubules in living S. pombe. In vitro, Klp5 and 6 dimers exhibit a hitherto-undescribed combination of strong enhancement of microtubule nucleation with no effect on growth rate or catastrophe frequency. By contrast in vivo, both Klp5 and Klp6 promote microtubule catastrophe at cell ends whilst Klp6 also increases the number of interphase microtubule arrays (IMAs). Our data support a model in which Klp5/6 bind tightly to free tubulin heterodimers, strongly promoting the nucleation of new microtubules, and then continue to land as a tubulin-motor complex on the tips of growing microtubules, with the motors then dissociating after a few seconds residence on the lattice. In vivo, we predict that only at cell ends, when growing microtubule tips become lodged and their growth slows down, will Klp5/6 motor activity succeed in tracking growing microtubule tips. This mechanism would allow Klp5/6 to detect the arrival of microtubule tips at cells ends and to amplify the intrinsic tendency for microtubules to catastrophise in compression at cell ends. Our evidence identifies Klp5 and 6 as spatial regulators of microtubule dynamics that enhance both microtubule nucleation at the cell centre and microtubule catastrophe at the cell ends.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.