ArticlePloS one2012
Molecular cloning and characterization of two genes encoding dihydroflavonol-4-reductase from Populus trichocarpa.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 52 citations in OpenAlex.
- The role of RaDFR2 regulating flower color formation in Rhododendron agastum.BMC plant biology · 2026Article
- Multi-omics analysis provides insights into the mechanism underlying fruit color formation inFrontiers in plant science · 2024Article
- Total Flavonoid Contents and the Expression of Flavonoid Biosynthetic Genes in Breadfruit (Plants (Basel, Switzerland) · 2023Article
- Cloning and functional analysis of GhDFR1, a key gene of flavonoid synthesis pathway in naturally colored cotton.Molecular biology reports · 2023Article
- Biosynthesis and regulation of anthocyanin pathway genes.Applied microbiology and biotechnology · 2022Review
- Functional Analysis of GenesBioMed research international · 2022Article
- Comparative transcriptome analysis reveals key genes associated with pigmentation in radish (Raphanus sativus L.) skin and flesh.Scientific reports · 2021Article
- Insights of Phenolic Pathway in Fruits: Transcriptional and Metabolic Profiling in Apricot (International journal of molecular sciences · 2021Article
- Article
- Cloning and Functional Characterization of Dihydroflavonol 4-Reductase Gene Involved in Anthocyanin Biosynthesis of Chrysanthemum.International journal of molecular sciences · 2020Article
- Review
- Metabolome and Transcriptome Analysis Reveals Putative Genes Involved in Anthocyanin Accumulation and Coloration in White and Pink Tea (Molecules (Basel, Switzerland) · 2020Article
- Transcriptome profiling reveals key genes related to astringency during cucumber fruit development.3 Biotech · 2019Article
- Comparative transcription analysis of photosensitive and non-photosensitive eggplants to identify genes involved in dark regulated anthocyanin synthesis.BMC genomics · 2019Article
- Metabolome and Transcriptome Sequencing Analysis Reveals Anthocyanin Metabolism in Pink Flowers of Anthocyanin-Rich Tea (Molecules (Basel, Switzerland) · 2019Article
- Integrating Transcriptomic and GC-MS Metabolomic Analysis to Characterize Color and Aroma Formation during Tepal Development inPlants (Basel, Switzerland) · 2019Article
- Comprehensive analysis of putative dihydroflavonol 4-reductase gene family in tea plant.PloS one · 2019Article
- Effect of Red and Blue Light on Anthocyanin Accumulation and Differential Gene Expression in Strawberry (Fragaria × ananassa).Molecules (Basel, Switzerland) · 2018Article
- Article
- A Root-Preferential DFR-Like Gene Encoding Dihydrokaempferol Reductase Involved in Anthocyanin Biosynthesis of Purple-Fleshed Sweet Potato.Frontiers in plant science · 2017Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dihydroflavonol 4-reductase (DFR, EC 1.1.1.219) is a rate-limited enzyme in the biosynthesis of anthocyanins and condensed tannins (proanthocyanidins) that catalyzes the reduction of dihydroflavonols to leucoanthocyanins. In this study, two full-length transcripts encoding for PtrDFR1 and PtrDFR2 were isolated from Populus trichocarpa. Sequence alignment of the two PtrDFRs with other known DFRs reveals the homology of these genes. The expression profile of PtrDFRs was investigated in various tissues of P. trichocarpa. To determine their functions, two PtrDFRs were overexpressed in tobacco (Nicotiana tabacum) via Agrobacterium-mediated transformation. The associated color change in the flowers was observed in all 35S:PtrDFR1 lines, but not in 35S:PtrDFR2 lines. Compared to the wild-type control, a significantly higher accumulation of anthocyanins was detected in transgenic plants harboring the PtrDFR1. Furthermore, overexpressing PtrDFR1 in Chinese white poplar (P. tomentosa Carr.) resulted in a higher accumulation of both anthocyanins and condensed tannins, whereas constitutively expressing PtrDFR2 only improved condensed tannin accumulation, indicating the potential regulation of condensed tannins by PtrDFR2 in the biosynthetic pathway in poplars.
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