ArticleMolecular breeding : new strategies in plant improvement2012
High-resolution melting analysis for SNP genotyping and mapping in tetraploid alfalfa (Medicago sativa L.).
Article in Molecular breeding : new strategies in plant improvement, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 77 citations in OpenAlex.
- Identification, validation and candidate gene analysis of major QTL for Supernumerary spikelets in wheat.BMC genomics · 2024Article
- Comparison of genotyping assays for detection of targeted CRISPR/Cas mutagenesis in highly polyploid sugarcane.Frontiers in genome editing · 2024Article
- Portuguese wild hop diversity assessment by fast SNP genotyping using high-resolution melting.Journal of applied genetics · 2022Article
- Construction of a high-density genetic map and localization of grazing-tolerant QTLs inFrontiers in plant science · 2022Article
- Improving Quantitative Power in Digital PCR through Digital High-Resolution Melting.Journal of clinical microbiology · 2020Article
- A High Resolution Melting Analysis-Based Genotyping Toolkit for the Peach (International journal of molecular sciences · 2020Article
- Flexibility and thermal dynamic stability increase of dsDNA induced by Ru(bpy)BMC chemistry · 2019Article
- DNA Mini-Barcoding: A Derived Barcoding Method for Herbal Molecular Identification.Frontiers in plant science · 2019Review
- Haplotype analysis of the germacrene A synthase gene and association with cynaropicrin content and biological activities in Cynara cardunculus.Molecular genetics and genomics : MGG · 2018Article
- A novel and quick PCR-based method to genotype mice with a leptin receptor mutation (db/db mice).Acta pharmacologica Sinica · 2018Article
- The development of functional mapping by three sex-related loci on the third whorl of different sex types of Carica papaya L.PloS one · 2018Article
- Article
- A Novel Prokaryotic Green Fluorescent Protein Expression System for Testing Gene Editing Tools Activity Like Zinc Finger Nuclease.Advanced biomedical research · 2017Article
- Genotyping-by-Sequencing and Its Exploitation for Forage and Cool-Season Grain Legume Breeding.Frontiers in plant science · 2017Article
- Mapping and validation of major quantitative trait loci for kernel length in wild barley (Hordeum vulgare ssp. spontaneum).BMC genetics · 2016Article
- Identification and validation of novel low-tiller number QTL in common wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- Assessment for Melting Temperature Measurement of Nucleic Acid by HRM.Journal of analytical methods in chemistry · 2016Article
- Article
- Anchoring linkage groups of the Rosa genetic map to physical chromosomes with tyramide-FISH and EST-SNP markers.PloS one · 2014Article
- Linkage analysis and QTL mapping using SNP dosage data in a tetraploid potato mapping population.PloS one · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single nucleotide polymorphisms (SNPs) represent the most abundant type of genetic polymorphism in plant genomes. SNP markers are valuable tools for genetic analysis of complex traits of agronomic importance, linkage and association mapping, genome-wide selection, map-based cloning, and marker-assisted selection. Current challenges for SNP genotyping in polyploid outcrossing species include multiple alleles per loci and lack of high-throughput methods suitable for variant detection. In this study, we report on a high-resolution melting (HRM) analysis system for SNP genotyping and mapping in outcrossing tetraploid genotypes. The sensitivity and utility of this technology is demonstrated by identification of the parental genotypes and segregating progeny in six alfalfa populations based on unique melting curve profiles due to differences in allelic composition at one or multiple loci. HRM using a 384-well format is a fast, consistent, and efficient approach for SNP discovery and genotyping, useful in polyploid species with uncharacterized genomes. Possible applications of this method include variation discovery, analysis of candidate genes, genotyping for comparative and association mapping, and integration of genome-wide selection in breeding programs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-011-9566-x) contains supplementary material, which is available to authorized users.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.