ArticlePlant physiology2012
A revised architecture of primary cell walls based on biomechanical changes induced by substrate-specific endoglucanases.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 147 papers.
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Who cites it
147 citing papers in PubMed, 396 citations in OpenAlex.
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- Revealing structure and shaping priorities in plant and fungal cell wall architecture via solid-state NMR.Cell surface (Amsterdam, Netherlands) · 2025Review
- Advances in Cell Wall Dynamics and Gene Expression in Postharvest Fruit Softening.Plants (Basel, Switzerland) · 2025Review
- Fibrous network nature of plant cell walls enables tunable mechanics for development.Nature communications · 2025Article
- Morphology, histochemistry and ultrastructure of the floral glandular trichomes in three Doronicum species (Asteraceae).Protoplasma · 2025Article
- Ethylene response factors as potential regulators of cell wall formation in celery (Apium graveolens L.) collenchyma: phylogeny and gene expression analysis.Protoplasma · 2025Article
- QTL mapping and transcriptome analysis identified BnaA03.XTH4 as a novel negative regulator of plant height in Brassica napus L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
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- Time-resolved tracking of cellulose biosynthesis and assembly during cell wall regeneration in liveScience advances · 2025Article
- SANS investigation of fungal loosenins reveals substrate-dependent impacts of protein action on the inter-microfibril arrangement of cellulosic substrates.Biotechnology for biofuels and bioproducts · 2025Article
- The effect of hemicelluloses on biosynthesis, structure and mechanical performance of bacterial cellulose-hemicellulose hydrogels.Scientific reports · 2024Article
- CELLULOSE SYNTHASE-LIKE C proteins modulate cell wall establishment during ethylene-mediated root growth inhibition in rice.The Plant cell · 2024Article
- Xyloglucan deficiency leads to a reduction in turgor pressure and changes in cell wall properties, affecting early seedling establishment.Current biology : CB · 2024Article
- Xyloglucan-Cellulose Nanocrystals Mixtures: A Case Study of Nanocolloidal Hydrogels and Levers for Tuning Functional Properties.Gels (Basel, Switzerland) · 2024Article
- Identification and expression analysis of the Xyloglucan transglycosylase/hydrolase (XTH) gene family under abiotic stress in oilseed (Brassica napus L.).BMC plant biology · 2024Article
- Structure and growth of plant cell walls.Nature reviews. Molecular cell biology · 2024Review
- The plant cell wall-dynamic, strong, and adaptable-is a natural shapeshifter.The Plant cell · 2024Review
87 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xyloglucan is widely believed to function as a tether between cellulose microfibrils in the primary cell wall, limiting cell enlargement by restricting the ability of microfibrils to separate laterally. To test the biomechanical predictions of this "tethered network" model, we assessed the ability of cucumber (Cucumis sativus) hypocotyl walls to undergo creep (long-term, irreversible extension) in response to three family-12 endo-β-1,4-glucanases that can specifically hydrolyze xyloglucan, cellulose, or both. Xyloglucan-specific endoglucanase (XEG from Aspergillus aculeatus) failed to induce cell wall creep, whereas an endoglucanase that hydrolyzes both xyloglucan and cellulose (Cel12A from Hypocrea jecorina) induced a high creep rate. A cellulose-specific endoglucanase (CEG from Aspergillus niger) did not cause cell wall creep, either by itself or in combination with XEG. Tests with additional enzymes, including a family-5 endoglucanase, confirmed the conclusion that to cause creep, endoglucanases must cut both xyloglucan and cellulose. Similar results were obtained with measurements of elastic and plastic compliance. Both XEG and Cel12A hydrolyzed xyloglucan in intact walls, but Cel12A could hydrolyze a minor xyloglucan compartment recalcitrant to XEG digestion. Xyloglucan involvement in these enzyme responses was confirmed by experiments with Arabidopsis (Arabidopsis thaliana) hypocotyls, where Cel12A induced creep in wild-type but not in xyloglucan-deficient (xxt1/xxt2) walls. Our results are incompatible with the common depiction of xyloglucan as a load-bearing tether spanning the 20- to 40-nm spacing between cellulose microfibrils, but they do implicate a minor xyloglucan component in wall mechanics. The structurally important xyloglucan may be located in limited regions of tight contact between microfibrils.
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