ArticleProceedings of the National Academy of Sciences of the United States of America1990
Actin cores of hair-cell stereocilia support myosin motility.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 45 citations in OpenAlex.
- Cy3-ATP labeling of unfixed, permeabilized mouse hair cells.Scientific reports · 2021Article
- Electron cryo-tomography of vestibular hair-cell stereocilia.Journal of structural biology · 2019Article
- Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid.The Journal of cell biology · 2016Article
- Molecular remodeling of tip links underlies mechanosensory regeneration in auditory hair cells.PLoS biology · 2013Article
- Article
- Sliding adhesion confers coherent motion to hair cell stereocilia and parallel gating to transduction channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Distribution of frequencies of spontaneous oscillations in hair cells of the bullfrog sacculus.Biophysical journal · 2009Article
- Protein localization by actin treadmilling and molecular motors regulates stereocilia shape and treadmilling rate.Biophysical journal · 2008Article
- An actin molecular treadmill and myosins maintain stereocilia functional architecture and self-renewal.The Journal of cell biology · 2004Article
- Resource Letter: LBOT-1: Laser-based optical tweezers.American journal of physics · 2003Article
- The optical stretcher: a novel laser tool to micromanipulate cells.Biophysical journal · 2001Article
- Two mechanisms for transducer adaptation in vertebrate hair cells.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Unconventional myosins in inner-ear sensory epithelia.The Journal of cell biology · 1997Article
- Adenine nucleoside diphosphates block adaptation of mechanoelectrical transduction in hair cells.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The actin cores of hair-cell stereocilia were tested as a substrate for the movement of myosin-coated beads in an in vitro assay. Large numbers of stereocilia from bullfrog sacculi and semicircular canals were isolated by blotting onto coverglasses and were demembranated to expose the polar actin tracks of their cytoskeletal cores. Silica or polystyrene beads, coated with thick filaments of chicken skeletal muscle myosin, were added to this core preparation in the presence of ATP. Myosin-coated beads could reach some of the cores by diffusion alone, but the efficiency and precision of the assay were improved considerably by the use of "optical tweezers" (a gradient-force optical trap) to deposit the beads directly on the cores. Beads applied in this fashion bound and moved unidirectionally at 1-2 microns/s, escaping the retarding force of the trap. Actin filaments within the stereocilia are cross-linked by fimbrin, but this did not appear to interfere with the motility of myosin. Beads coated with optic-lobe kinesin were also tested for movement; these bound and moved unidirectionally at 0.1-0.2 microns/s when applied to microtubule-based kinociliary cores, but not when applied to actin-based stereociliary cores. Our results are consistent with, and lend support to, a model for hair cell adaptation in which a molecular motor such as myosin maintains tension on the mechanically gated transduction channels. Optical tweezers and video-enhanced differential interference contrast optics provide high efficiency and improved optical resolution for the in vitro analysis of myosin motility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.