ArticleProceedings of the National Academy of Sciences of the United States of America1990
A 47-kDa human nuclear protein recognized by antikinetochore autoimmune sera is homologous with the protein encoded by RCC1, a gene implicated in onset of chromosome condensation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 94 citations in OpenAlex.
- Article
- RCC1 regulates inner centromeric composition in a Ran-independent fashion.Cell cycle (Georgetown, Tex.) · 2018Article
- A pull-down procedure for the identification of unknown GEFs for small GTPases.Small GTPases · 2016Article
- Review
- Centromere activity in dicentric small supernumerary marker chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Ran GTPase guanine nucleotide exchange factor RCC1 is phosphorylated on serine 11 by cdc2 kinase in vitro.Molecular biology reports · 2009Article
- Fine specificity mapping of autoantigens targeted by anti-centromere autoantibodies.Journal of autoimmunity · 2006Article
- Kinetochore-driven formation of kinetochore fibers contributes to spindle assembly during animal mitosis.The Journal of cell biology · 2004Article
- Detection of anaphylatoxin receptors on CD83+ dendritic cells derived from human skin.Immunology · 2001Article
- A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.The Journal of cell biology · 1996Article
- Cellular expression of human centromere protein C demonstrates a cyclic behavior with highest abundance in the G1 phase.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Inactivation of the yeast Sen1 protein affects the localization of nucleolar proteins.Molecular & general genetics : MGG · 1995Article
- Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1.The EMBO journal · 1995Article
- A tightly bound chromosome antigen is detected by monoclonal antibodies in a ring-like structure on human centromeres.Chromosoma · 1995Article
- Isolation of chromosome-associated proteins from Drosophila melanogaster that bind a human centromeric DNA sequence.The Journal of cell biology · 1994Article
- Effects of mutant Ran/TC4 proteins on cell cycle progression.Molecular and cellular biology · 1994Article
- Human autoimmune sera recognize a conserved 26 kD protein associated with mammalian heterochromatin that is homologous to heterochromatin protein 1 of Drosophila.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1994Article
- RanGAP1 induces GTPase activity of nuclear Ras-related Ran.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Sequence of a plant cDNA from Vicia faba encoding a novel Ran-related GTP-binding protein.Plant molecular biology · 1994Article
- A stable dicentric chromosome: both centromeres develop kinetochores and attach to the spindle in monocentric and dicentric configuration.Chromosoma · 1994Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several autoimmune sera from patients with Raynaud phenomenon decorated mammalian kinetochores and bound to a 47-kDa protein on immunoblots of nuclear lysates. Antibody affinity-purified from immunoblots of the 47-kDa band recognized kinetochores, but due to crossreaction with an 18-kDa protein, localization remains elusive. We used one of these sera to purify the antigen from HeLa cells synchronized in mitosis as a noncovalent complex with a 25-kDa protein. The antigen was released from DNA by intercalation with 25 mM chloroquine. Ion-exchange chromatography yielded the pure complex with an apparent molecular size of 68 kDa, which was separated into its components by gel filtration in 6 M guanidinium chloride. Upon two-dimensional gel electrophoresis the 47-kDa protein gave two main spots of pI 6.6 and 6.7, respectively. Posttranslational modification is indicated by additional antigenic spots, by lack of a free alpha-amino group, and by chromatographic behavior of peptides on reversed-phase chromatography. The amino acid sequence for 205 residues of the 47-kDa protein has been established. This sequence is highly homologous with the translated reading frame of RCC1, a gene reportedly involved in regulating onset of mammalian chromosome condensation.
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