ArticleThe Biochemical journal2012
Heat-shock proteins attenuate SERCA inactivation by the anti-apoptotic protein Bcl-2: possible implications for the ER Ca2+-mediated apoptosis.
Article in The Biochemical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- DNAJB3/HSP-40 cochaperone is downregulated in obese humans and is restored by physical exercise.PloS one · 2013Trial
- Time-series analysis of gene expression identifies of FKBP4 as a novel protective factor in severe acute pancreatitis-associated myocardial injury.Biochemistry and biophysics reports · 2026Article
- Isolation of the Sarcoplasmic Reticulum CaMethods and protocols · 2023Article
- Active nNOS Is Required for Grp94-Induced Antioxidant Cytoprotection: A Lesson from Myogenic to Cancer Cells.International journal of molecular sciences · 2022Article
- Modulation of Heat-Shock Proteins Mediates Chicken Cell Survival against Thermal Stress.Animals : an open access journal from MDPI · 2020Review
- Targeting Bcl-2-IPBiochimica et biophysica acta. Molecular cell research · 2018Review
- SERCA control of cell death and survival.Cell calcium · 2018Review
- Anticancer Effects of Dihydroartemisinin on Human Esophageal Cancer Cells In Vivo.Analytical cellular pathology (Amsterdam) · 2018Article
- Crosstalk between calcium and reactive oxygen species signaling in cancer.Cell calcium · 2017Review
- Inhibition and conformational change of SERCA3b induced by Bcl-2.Biochimica et biophysica acta. Proteins and proteomics · 2017Article
- Regulation of Endoplasmic Reticulum-Mitochondria CaFrontiers in oncology · 2017Review
- Elucidating a molecular mechanism that the deterioration of porcine meat quality responds to increased cortisol based on transcriptome sequencing.Scientific reports · 2016Article
- Review
- Heat shock protein 70 protects PC12 cells against ischemia-hypoxia/reoxygenation by maintaining intracellular Ca(2+) homeostasis.Neural regeneration research · 2016Article
- Bcl-2 regulation of the inositol 1,4,5-trisphosphate receptor and calcium signaling in normal and malignant lymphocytes: potential new target for cancer treatment.Biochimica et biophysica acta · 2014Review
- Combined effects of astragalus soup and persistent Taiji boxing on improving the immunity of elderly women.International journal of clinical and experimental medicine · 2014Article
- Apoptosis in differentiating C2C12 muscle cells selectively targets Bcl-2-deficient myotubes.Apoptosis : an international journal on programmed cell death · 2014Article
- The selective BH4-domain biology of Bcl-2-family members: IP3Rs and beyond.Cellular and molecular life sciences : CMLS · 2013Review
- Alpha-helical destabilization of the Bcl-2-BH4-domain peptide abolishes its ability to inhibit the IP3 receptor.PloS one · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have demonstrated previously that Bcl-2 and Bcl-2Δ21, a C-terminally truncated Bcl-2 sequence, inactivate SERCA (sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase) 1 in isolated SR (sarcoplasmic reticulum), accompanied by a translocation from CRDs (caveolae-related domains) of the SR. In the present study, we obtained evidence for the interaction of Bcl-2 with SERCA2b in C2C12 myoblasts and HEK (human embryonic kidney)-293 cells. Bcl-2 and SERCA2b co-immunoprecipitated from lysate and microsomal fractions of Bcl-2-overexpressing cells. However, Bcl-2 overexpression resulted only in a slight translocation from the CRDs and no significant SERCA inactivation. In isolated HEK-293 cell microsomes, incubation with Bcl-2Δ21 afforded SERCA2b inactivation and some translocation. HSP (heat-shock protein) 70, HSP90, HSP27 and α-crystallin attenuated Bcl-2Δ21-dependent SERCA2b inactivation. An in vitro mechanistic study with the SERCA1 isoform shows that HSP70 (i) protects SERCA1 from the inactivation by Bcl-2Δ21, (ii) inhibits SERCA1 translocation from CRD fractions, and (iii) prevents the Bcl-2Δ21-dependent loss of FITC labelling. Our data demonstrate that the mechanism of SERCA inactivation by Bcl-2 established in vitro for the SERCA1 isoform can be extended to the main housekeeping SERCA2b isoform, and that functional interactions of SERCA2b and Bcl-2 in the cell may be modulated by HSP70 and other chaperones and stress-regulated proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.