ArticlePloS one2012
Fourier transform infrared spectroscopic imaging and multivariate regression for prediction of proteoglycan content of articular cartilage.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- Degeneration dependent changes in human knee cartilage mechanical properties revealed by synchrotron tomography based finite element modeling.Osteoarthritis and cartilage open · 2026Article
- Spatial Raman Spectroscopy to Characterize (Sulfated) Glycosaminoglycans in Human Articular Cartilage.International journal of molecular sciences · 2025Article
- Climate history modulates stress responses of common soil bacteria under experimental drought.The ISME journal · 2025Article
- Impact of storage techniques on ovine temporomandibular joint discs composition and physicochemical properties.Frontiers in bioengineering and biotechnology · 2025Article
- Protein Analysis by FT-IR/ATR Spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Preclassification of Broadband and Sparse Infrared Data by Multiplicative Signal Correction Approach.Molecules (Basel, Switzerland) · 2022Article
- Applications of Vibrational Spectroscopy for Analysis of Connective Tissues.Molecules (Basel, Switzerland) · 2021Review
- The synovial surface of the articular cartilage.European journal of histochemistry : EJH · 2020Article
- The use of Fourier-transform infrared spectroscopy to characterize connective tissue components in skeletal muscle of Atlantic cod (Gadus morhua L.).Journal of biophotonics · 2019Article
- Composition, structure and tensile biomechanical properties of equine articular cartilage during growth and maturation.Scientific reports · 2018Article
- Vibrational spectroscopic techniques to assess bone quality.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2017Review
- Heparin/Collagen 3D Scaffold Accelerates Hepatocyte Differentiation of Wharton's Jelly-Derived Mesenchymal Stem Cells.Tissue engineering and regenerative medicine · 2017Article
- Imaging of Osteoarthritic Human Articular Cartilage using Fourier Transform Infrared Microspectroscopy Combined with Multivariate and Univariate Analysis.Scientific reports · 2016Article
- Comparative Study of Morphometric and Fourier Transform Infrared Spectroscopy Analyses of the Collagen Fibers in the Repair Process of Cutaneous Lesions.Advances in wound care · 2016Article
- Microfluidic approaches to synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectral microscopy of living biosystems.Protein and peptide letters · 2016Article
- Proteoglycan concentrations in healthy and diseased articular cartilage by Fourier transform infrared imaging and principal component regression.Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy · 2014Article
- FTIR-I compositional mapping of the cartilage-to-bone interface as a function of tissue region and age.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2014Article
- Vibrational spectroscopic imaging for the evaluation of matrix and mineral chemistry.Current osteoporosis reports · 2014Review
- Structure-function relations and rigidity percolation in the shear properties of articular cartilage.Biophysical journal · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fourier Transform Infrared (FT-IR) spectroscopic imaging has been earlier applied for the spatial estimation of the collagen and the proteoglycan (PG) contents of articular cartilage (AC). However, earlier studies have been limited to the use of univariate analysis techniques. Current analysis methods lack the needed specificity for collagen and PGs. The aim of the present study was to evaluate the suitability of partial least squares regression (PLSR) and principal component regression (PCR) methods for the analysis of the PG content of AC. Multivariate regression models were compared with earlier used univariate methods and tested with a sample material consisting of healthy and enzymatically degraded steer AC. Chondroitinase ABC enzyme was used to increase the variation in PG content levels as compared to intact AC. Digital densitometric measurements of Safranin O-stained sections provided the reference for PG content. The results showed that multivariate regression models predict PG content of AC significantly better than earlier used absorbance spectrum (i.e. the area of carbohydrate region with or without amide I normalization) or second derivative spectrum univariate parameters. Increased molecular specificity favours the use of multivariate regression models, but they require more knowledge of chemometric analysis and extended laboratory resources for gathering reference data for establishing the models. When true molecular specificity is required, the multivariate models should be used.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.