ArticlePloS one2012
Genome organization, phylogenies, expression patterns, and three-dimensional protein models of two acetylcholinesterase genes from the red flour beetle.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 53 citations in OpenAlex.
- Molecular Docking of Detoxification Enzymes fromBiology · 2025Article
- Protection of insect neurons by erythropoietin/CRLF3-mediated regulation of pro-apoptotic acetylcholinesterase.Scientific reports · 2022Article
- Role of Bmal1 in mediating the cholinergic system to regulate the behavioral rhythm of nocturnal marine molluscs.Computational and structural biotechnology journal · 2022Article
- Comparative analyses of transcriptional responses of Dectes texanus LeConte (Coleoptera: Cerambycidae) larvae fed on three different host plants and artificial diet.Scientific reports · 2021Article
- Article
- Molecular Characterization and Expression Analysis of Two Acetylcholinesterase Genes From the Small White Butterfly Pieris rapae (Lepidoptera: Pieridae).Journal of insect science (Online) · 2018Article
- Neurotoxic Effects of Linalool and β-Pinene on Tribolium castaneum Herbst.Molecules (Basel, Switzerland) · 2017Article
- Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways.Scientific reports · 2017Article
- Toxicity of Naphthalene and Benzene on Tribollium castaneum Herbst.International journal of environmental research and public health · 2017Article
- Expression of acetylcholinesterase 1 is associated with brood rearing status in the honey bee, Apis mellifera.Scientific reports · 2017Article
- Article
- The lethal giant larvae gene in Tribolium castaneum: molecular properties and roles in larval and pupal development as revealed by RNA interference.International journal of molecular sciences · 2014Article
- Identification and validation of reference genes for quantitative real-time PCR in Drosophila suzukii (Diptera: Drosophilidae).PloS one · 2014Article
- Biochemical properties, expression profiles, and tissue localization of orthologous acetylcholinesterase-2 in the mosquito, Anopheles gambiae.Insect biochemistry and molecular biology · 2013Article
- Novel selective and irreversible mosquito acetylcholinesterase inhibitors for controlling malaria and other mosquito-borne diseases.Scientific reports · 2013Article
- Article
- Testing insecticidal activity of novel chemically synthesized siRNA against Plutella xylostella under laboratory and field conditions.PloS one · 2013Article
- Transcriptome profiling of Chironomus kiinensis under phenol stress using Solexa sequencing technology.PloS one · 2013Article
- Molecular and kinetic properties of two acetylcholinesterases from the western honey bee, Apis mellifera.PloS one · 2012Article
- Cholinergic and non-cholinergic functions of two acetylcholinesterase genes revealed by gene-silencing in Tribolium castaneum.Scientific reports · 2012Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since the report of a paralogous acetylcholinesterase (AChE, EC3.1.1.7) gene in the greenbug (Schizaphis graminum) in 2002, two different AChE genes (Ace1 and Ace2) have been identified in each of at least 27 insect species. However, the gene models of Ace1 and Ace2, and their molecular properties have not yet been comprehensively analyzed in any insect species. In this study, we sequenced the full-length cDNAs, computationally predicted the corresponding three-dimensional protein models, and profiled developmental stage and tissue-specific expression patterns of two Ace genes from the red flour beetle (Tribolium castaneum; TcAce1 and TcAce2), a globally distributed major pest of stored grain products and an emerging model organism. TcAce1 and TcAce2 encode 648 and 604 amino acid residues, respectively, and have conserved motifs including a choline-binding site, a catalytic triad, and an acyl pocket. Phylogenetic analysis show that both TcAce genes are grouped into two insect Ace clusters and TcAce1 is completely diverged from TcAce2, suggesting that these two genes evolve from their corresponding Ace gene lineages in insect species. In addition, TcAce1 is located on chromosome 5, whereas TcAce2 is located on chromosome 2. Reverse transcription polymerase chain reaction (PCR) and quantitative real-time PCR analyses indicate that both genes are virtually transcribed in all the developmental stages and predominately expressed in the insect brain. Our computational analyses suggest that the TcAce1 protein is a robust acetylcholine (ACh) hydrolase and has susceptibility to sulfhydryl agents whereas the TcAce2 protein is not a catalytically efficient ACh hydrolase.
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