ArticlePloS one2012
Shape and compliance of endothelial cells after shear stress in vitro or from different aortic regions: scanning ion conductance microscopy study.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Spatial regulation of hydrogel polymerization reaction using ultrasound-driven streaming vortex.Ultrasonics sonochemistry · 2024Article
- Review
- Effects of Pulsatility on Arterial Endothelial and Smooth Muscle Cells.Cells, tissues, organs · 2023Article
- Scanning Ion Conductance Microscopy.Chemical reviews · 2021Review
- Current understanding of intimal hyperplasia and effect of compliance in synthetic small diameter vascular grafts.Biomaterials science · 2020Review
- Endothelial Cell Biomechanical Responses are Dependent on Both Fluid Shear Stress and Tensile Strain.Cellular and molecular bioengineering · 2019Article
- Understanding mechanobiology in cultured endothelium: A review of the orbital shaker method.Atherosclerosis · 2019Review
- Increased MMP activity in curved geometries disrupts the endothelial cell glycocalyx creating a proinflammatory environment.PloS one · 2018Article
- Visualization of three pathways for macromolecule transport across cultured endothelium and their modification by flow.American journal of physiology. Heart and circulatory physiology · 2017Article
- 4-D Flow Control in Porous Scaffolds: Toward a Next Generation of Bioreactors.IEEE transactions on bio-medical engineering · 2017Article
- Change of direction in the biomechanics of atherosclerosis.Annals of biomedical engineering · 2015Review
- Cellular and pharmacological targets to induce coronary arteriogenesis.Current cardiology reviews · 2014Review
- Patterned cell culture substrates created by hot embossing of tissue culture treated polystyrene.Journal of materials science. Materials in medicine · 2013Article
- Scanning ion conductance microscopy for studying biological samples.Sensors (Basel, Switzerland) · 2012Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
objectiveTo measure the elongation and compliance of endothelial cells subjected to different patterns of shear stress in vitro, and to compare these parameters with the elongation and compliance of endothelial cells from different regions of the intact aorta. MATERIALS AND
methodsPorcine aortic endothelial cells were cultured for 6 days under static conditions or on an orbital shaker. The shaker generated a wave of medium, inducing pulsatile shear stress with a preferred orientation at the edge of the well or steadier shear stress with changing orientation at its centre. The topography and compliance of these cells and cells from the inner and outer curvature of ex vivo porcine aortic arches were measured by scanning ion conductance microscopy (SICM).
resultsCells cultured under oriented shear stress were more elongated and less compliant than cells grown under static conditions or under shear stress with no preferred orientation. Cells from the outer curvature of the aorta were more elongated and less compliant than cells from the inner curvature.
conclusionThe elongation and compliance of cultured endothelial cells vary according to the pattern of applied shear stress, and are inversely correlated. A similar inverse correlation occurs in the aortic arch, with variation between regions thought to experience different haemodynamic stresses.
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