ArticlePloS one2012
The role of actin turnover in retrograde actin network flow in neuronal growth cones.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 69 citations in OpenAlex.
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- Phenotypic Heterogeneity in Titinopathies with Peripheral Nerve Involvement in Pediatric Age: Two Case Reports.Journal of clinical medicine · 2026Article
- SiR-XActin: A Fluorescent Probe for Imaging Actin Dynamics in Live Cells.Angewandte Chemie (International ed. in English) · 2025Article
- Bacterial Extracellular Vesicles Exploit Filopodial Surfing and Retraction Mechanisms to Reach the Host Cell Body in an Actin-Dependent Manner.Journal of extracellular vesicles · 2025Article
- Piezo1 Regulates Stiffness-Dependent DRG Axon Regeneration via Modifying Cytoskeletal Dynamics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Modeling the dynamics of actin and myosin during bleb stabilization.bioRxiv : the preprint server for biology · 2023Article
- Computational simulations reveal that Abl activity controls cohesiveness of actin networks in growth cones.Molecular biology of the cell · 2022Article
- Pulling, failing, and adaptive mechanotransduction of macrophage filopodia.Biophysical journal · 2022Article
- Actin polymerization state regulates osteogenic differentiation in human adipose-derived stem cells.Cellular & molecular biology letters · 2021Article
- A binding protein regulates myosin-7a dimerization and actin bundle assembly.Nature communications · 2021Article
- Microtubule Growth Rates Are Sensitive to Global and Local Changes in Microtubule Plus-End Density.Current biology : CB · 2020Article
- Arp2/3 and Mena/VASP Require Profilin 1 for Actin Network Assembly at the Leading Edge.Current biology : CB · 2020Article
- Axon growth and synaptic function: A balancing act for axonal regeneration and neuronal circuit formation in CNS trauma and disease.Developmental neurobiology · 2020Review
- Vangl2 acts at the interface between actin and N-cadherin to modulate mammalian neuronal outgrowth.eLife · 2020Article
- Article
- Plastic Deformation and Fragmentation of Strained Actin Filaments.Biophysical journal · 2019Article
- Remarkable structural transformations of actin bundles are driven by their initial polarity, motor activity, crosslinking, and filament treadmilling.PLoS computational biology · 2019Article
- RHO-1 and the Rho GEF RHGF-1 interact with UNC-6/Netrin signaling to regulate growth cone protrusion and microtubule organization in Caenorhabditis elegans.PLoS genetics · 2019Article
- Model for Coordination of Microtubule and Actin Dynamics in Growth Cone Turning.Frontiers in cellular neuroscience · 2018Article
- An Integrated Cytoskeletal Model of Neurite Outgrowth.Frontiers in cellular neuroscience · 2018Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The balance of actin filament polymerization and depolymerization maintains a steady state network treadmill in neuronal growth cones essential for motility and guidance. Here we have investigated the connection between depolymerization and treadmilling dynamics. We show that polymerization-competent barbed ends are concentrated at the leading edge and depolymerization is distributed throughout the peripheral domain. We found a high-to-low G-actin gradient between peripheral and central domains. Inhibiting turnover with jasplakinolide collapsed this gradient and lowered leading edge barbed end density. Ultrastructural analysis showed dramatic reduction of leading edge actin filament density and filament accumulation in central regions. Live cell imaging revealed that the leading edge retracted even as retrograde actin flow rate decreased exponentially. Inhibition of myosin II activity before jasplakinolide treatment lowered baseline retrograde flow rates and prevented leading edge retraction. Myosin II activity preferentially affected filopodial bundle disassembly distinct from the global effects of jasplakinolide on network turnover. We propose that growth cone retraction following turnover inhibition resulted from the persistence of myosin II contractility even as leading edge assembly rates decreased. The buildup of actin filaments in central regions combined with monomer depletion and reduced polymerization from barbed ends suggests a mechanism for the observed exponential decay in actin retrograde flow. Our results show that growth cone motility is critically dependent on continuous disassembly of the peripheral actin network.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.