ArticlePloS one2012
Transcriptional profiling of disease-induced host responses in bovine tuberculosis and the identification of potential diagnostic biomarkers.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 62 citations in OpenAlex.
- Integrative genomics sheds light on the immunogenetics of tuberculosis in cattle.Communications biology · 2025Article
- Screening of mRNA markers in early bovine tuberculosis blood samples.Frontiers in veterinary science · 2024Article
- The genetic architecture of complete blood counts in lactating Holstein dairy cows.Frontiers in genetics · 2024Article
- Protein Levels of Pro-Inflammatory Cytokines and Chemokines as Biomarkers ofPathogens (Basel, Switzerland) · 2022Article
- Transcriptional Profiling of Early and Late Phases of Bovine Tuberculosis.Infection and immunity · 2022Article
- The Bovine Tuberculoid Granuloma.Pathogens (Basel, Switzerland) · 2022Review
- In-depth systems biological evaluation of bovine alveolar macrophages suggests novel insights into molecular mechanisms underlyingFrontiers in microbiology · 2022Article
- Th22 Cells Are a Major Contributor to the Mycobacterial CD4Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Cell-Mediated Immunological Biomarkers and Their Diagnostic Application in Livestock and Wildlife Infected WithFrontiers in immunology · 2021Review
- Identifying Bacterial and Host Factors Involved in the Interaction ofFrontiers in immunology · 2021Article
- RNA-Seq Transcriptome Analysis of Peripheral Blood From Cattle Infected WithFrontiers in veterinary science · 2021Article
- Heterogeneity of Pulmonary Granulomas in Cattle Experimentally Infected WithFrontiers in veterinary science · 2021Article
- Integrative Network Analysis of Predicted miRNA-Targets Regulating Expression of Immune Response Genes in Bovine Coronavirus Infection.Frontiers in genetics · 2020Article
- Biomarkers for Detecting Resilience against Mycobacterial Disease in Animals.Infection and immunity · 2019Review
- Far-off and close-up feeding levels affect immunological performance in grazing dairy cows during the transition period.Journal of animal science · 2019Article
- Inflammation responses in patients with pulmonary tuberculosis in an intensive care unit.Experimental and therapeutic medicine · 2018Article
- Experimental infection of cattle with Mycobacterium tuberculosis isolates shows the attenuation of the human tubercle bacillus for cattle.Scientific reports · 2018Article
- Biomarkers for Early Stages of Johne's Disease Infection and Immunization in Goats.Frontiers in microbiology · 2018Article
- CD4+ and γδ T Cells are the main Producers of IL-22 and IL-17A in Lymphocytes from Mycobacterium bovis-infected Cattle.Scientific reports · 2016Article
- Interleukin-17A as a Biomarker for Bovine Tuberculosis.Clinical and vaccine immunology : CVI · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine tuberculosis (bTb) remains a major and economically important disease of livestock. Improved ante-mortem diagnostic tools would help to underpin novel control strategies. The definition of biomarkers correlating with disease progression could have impact on the rational design of novel diagnostic approaches for bTb. We have used a murine bTb model to identify promising candidates in the host transcriptome post-infection. RNA from in vitro-stimulated splenocytes and lung cells from BALB/c mice infected aerogenically with Mycobacterium bovis were probed with high-density microarrays to identify possible biomarkers of disease. In antigen-stimulated splenocytes we found statistically significant differential regulation of 1109 genes early (3 days) after infection and 1134 at a later time-point post-infection (14 days). 618 of these genes were modulated at both time points. In lung cells, 282 genes were significantly modulated post-infection. Amongst the most strongly up-regulated genes were: granzyme A, granzyme B, cxcl9, interleukin-22, and ccr6. The expression of 14 out of the most up-regulated genes identified in the murine studies was evaluated using in vitro with antigen-stimulated PBMC from uninfected and naturally infected cattle. We show that the expression of cxcl9, cxcl10, granzyme A and interleukin-22 was significantly increased in PBMC from infected cattle compared to naïve animals following PPD stimulation in vitro. Thus, murine transcriptome analysis can be used to predict immunological responses in cattle allowing the prioritisation of CXCLl9, CXCL10, Granzyme A and IL-22 as potential additional readout systems for the ante-mortem diagnosis of bovine tuberculosis.
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