ArticlePloS one2012
Structural organization of DNA in chlorella viruses.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Infection cycles of viruses of the phylum Nucleocytoviricota.Nature reviews. Microbiology · 2026Review
- Diversity and taxonomy of giant algal viruses: chloroviruses lead the way.Journal of virology · 2026Review
- SMRT Sequencing of Paramecium Bursaria Chlorella Virus-1 Reveals Diverse Methylation Stability in Adenines Targeted by Restriction Modification Systems.Frontiers in microbiology · 2020Article
- Chloroviruses.Viruses · 2019Review
- Adenovirus major core protein condenses DNA in clusters and bundles, modulating genome release and capsid internal pressure.Nucleic acids research · 2019Article
- Chloroviruses Lure Hosts through Long-Distance Chemical Signaling.Journal of virology · 2019Article
- Cryopreservation of Paramecium bursaria Chlorella Virus-1 during an active infection cycle of its host.PloS one · 2019Article
- Algal Viruses: The (Atomic) Shape of Things to Come.Viruses · 2018Review
- Length-independent DNA packing into nanopore zero-mode waveguides for low-input DNA sequencing.Nature nanotechnology · 2017Article
- Role of Condensing Particles in Polymer Confinement: A Model for Virus-Packed "Minichromosomes".Biophysical journal · 2017Article
- Structural studies demonstrating a bacteriophage-like replication cycle of the eukaryote-infecting Paramecium bursaria chlorella virus-1.PLoS pathogens · 2017Article
- Structural Organization of Baculovirus Occlusion Bodies and Protective Role of Multilayered Polyhedron Envelope Protein.Food and environmental virology · 2016Article
- Synergistic effects of ATP and RNA binding to human DEAD-box protein DDX1.Nucleic acids research · 2015Article
- Paramecium bursaria chlorella virus 1 proteome reveals novel architectural and regulatory features of a giant virus.Journal of virology · 2012Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
Chlorella viruses have icosahedral capsids with an internal membrane enclosing their large dsDNA genomes and associated proteins. Their genomes are packaged in the particles with a predicted DNA density of ca. 0.2 bp nm(-3). Occasionally infection of an algal cell by an individual particle fails and the viral DNA is dynamically ejected from the capsid. This shows that the release of the DNA generates a force, which can aid in the transfer of the genome into the host in a successful infection. Imaging of ejected viral DNA indicates that it is intimately associated with proteins in a periodic fashion. The bulk of the protein particles detected by atomic force microscopy have a size of ∼60 kDa and two proteins (A278L and A282L) of about this size are among 6 basic putative DNA binding proteins found in a proteomic analysis of DNA binding proteins packaged in the virion. A combination of fluorescence images of ejected DNA and a bioinformatics analysis of the DNA reveal periodic patterns in the viral DNA. The periodic distribution of GC rich regions in the genome provides potential binding sites for basic proteins. This DNA/protein aggregation could be responsible for the periodic concentration of fluorescently labeled DNA observed in ejected viral DNA. Collectively the data indicate that the large chlorella viruses have a DNA packaging strategy that differs from bacteriophages; it involves proteins and share similarities to that of chromatin structure in eukaryotes.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.