ArticlePLoS pathogens2012
Introgression of ivermectin resistance genes into a susceptible Haemonchus contortus strain by multiple backcrossing.
Article in PLoS pathogens, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 78 citations in OpenAlex.
- Scientists' Warning on the Rapid Evolution of Parasites in the Anthropocene.Evolutionary applications · 2026Article
- Chromosomal genome assembly resolves drug resistance loci in the parasitic nematode Teladorsagia circumcincta.PLoS pathogens · 2025Article
- The Mitogenome of the Haecon-5 Strain ofInternational journal of molecular sciences · 2024Article
- Quantifying the fitness effects of resistance alleles with and without anthelmintic selection pressure using Caenorhabditis elegans.PLoS pathogens · 2024Article
- Anthelmintic resistance of gastrointestinal nematodes in cattle in Brazil and Argentina - current status and global perspectives.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2024Review
- Expression of transporter genes in anthelmintic resistant isolates of Haemonchus contortus.Genetics and molecular biology · 2024Article
- Allele specific PCR for a major marker of levamisole resistance in Haemonchus contortus.International journal for parasitology. Drugs and drug resistance · 2022Article
- Article
- Transgenic Expression ofInternational journal of molecular sciences · 2022Article
- Comparative analysis on transcriptomics of ivermectin resistant and susceptible strains of Haemonchus contortus.Parasites & vectors · 2022Article
- Genome-wide analysis of the response to ivermectin treatment by a Swedish field population of Haemonchus contortus.International journal for parasitology. Drugs and drug resistance · 2022Article
- Assessment of Avermectins-Induced Toxicity in Animals.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Evaluation of changes in drug susceptibility and population genetic structure in Haemonchus contortus following worm replacement as a means to reverse the impact of multiple-anthelmintic resistance on a sheep farm.International journal for parasitology. Drugs and drug resistance · 2021Article
- Complementary Approaches with Free-living and Parasitic Nematodes to Understanding Anthelmintic Resistance.Trends in parasitology · 2021Review
- Challenges and opportunities for the adoption of molecular diagnostics for anthelmintic resistance.International journal for parasitology. Drugs and drug resistance · 2020Article
- Suppression of hyaluronidase reduces invasion and establishment of Haemonchus contortus larvae in sheep.Veterinary research · 2020Article
- Recombinant elongation factor 1 alpha of Haemonchus contortus affects the functions of goat PBMCs.Parasite immunology · 2020Article
- Article
- Extreme-QTL mapping of monepantel resistance in Haemonchus contortus.Parasites & vectors · 2019Article
- Population genomic and evolutionary modelling analyses reveal a single major QTL for ivermectin drug resistance in the pathogenic nematode, Haemonchus contortus.BMC genomics · 2019Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Anthelmintic drug resistance in livestock parasites is already widespread and in recent years there has been an increasing level of anthelmintic drug selection pressure applied to parasitic nematode populations in humans leading to concerns regarding the emergence of resistance. However, most parasitic nematodes, particularly those of humans, are difficult experimental subjects making mechanistic studies of drug resistance extremely difficult. The small ruminant parasitic nematode Haemonchus contortus is a more amenable model system to study many aspects of parasite biology and investigate the basic mechanisms and genetics of anthelmintic drug resistance. Here we report the successful introgression of ivermectin resistance genes from two independent ivermectin resistant strains, MHco4(WRS) and MHco10(CAVR), into the susceptible genome reference strain MHco3(ISE) using a backcrossing approach. A panel of microsatellite markers were used to monitor the procedure. We demonstrated that after four rounds of backcrossing, worms that were phenotypically resistant to ivermectin had a similar genetic background to the susceptible reference strain based on the bulk genotyping with 18 microsatellite loci and individual genotyping with a sub-panel of 9 microsatellite loci. In addition, a single marker, Hcms8a20, showed evidence of genetic linkage to an ivermectin resistance-conferring locus providing a starting point for more detailed studies of this genomic region to identify the causal mutation(s). This work presents a novel genetic approach to study anthelmintic resistance and provides a "proof-of-concept" of the use of forward genetics in an important model strongylid parasite of relevance to human hookworms. The resulting strains provide valuable resources for candidate gene studies, whole genome approaches and for further genetic analysis to identify ivermectin resistance loci.
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