ArticleCytotechnology1998
Effect of culture temperature on a recombinant CHO cell line producing a C-terminal α-amidating enzyme.
Article in Cytotechnology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 108 citations in OpenAlex.
- Improvement of protein purity in etanercept production through process optimization in recombinant CHO cell-based transient gene expression system.Bioprocess and biosystems engineering · 2026Article
- Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalentsBiomaterials and biosystems · 2022Article
- Comparative study of commercial media to improve GMP manufacturing of recombinant human interferon β-1a by CHO cells in perfusion bioreactor.Cytotechnology · 2022Article
- Bioengineering Outlook on Cultivated Meat Production.Micromachines · 2022Review
- Revealing Key Determinants of Clonal Variation in Transgene Expression in Recombinant CHO Cells Using Targeted Genome Editing.ACS synthetic biology · 2018Article
- Mild hypothermia upregulates myc and xbp1s expression and improves anti-TNFα production in CHO cells.PloS one · 2018Article
- Dynamic metabolic flux analysis using B-splines to study the effects of temperature shift on CHO cell metabolism.Metabolic engineering communications · 2015Article
- Article
- Differential effect of culture temperature and specific growth rate on CHO cell behavior in chemostat culture.PloS one · 2014Article
- The effect of different media composition and temperatures on the production of recombinant human growth hormone by CHO cells.Research in pharmaceutical sciences · 2013Article
- Article
- The effects of microcarrier culture on recombinant CHO cells under biphasic hypothermic culture conditions.Cytotechnology · 2009Article
- The effects of culture conditions on the glycosylation of secreted human placental alkaline phosphatase produced in Chinese hamster ovary cells.Biotechnology and bioengineering · 2008Article
- Transcriptional profiling of gene expression changes in a PACE-transfected CHO DUKX cell line secreting high levels of rhBMP-2.Molecular biotechnology · 2008Article
- Differential protein expression following low temperature culture of suspension CHO-K1 cells.BMC biotechnology · 2008Article
- Article
- MicroRNAs: recently discovered key regulators of proliferation and apoptosis in animal cells : Identification of miRNAs regulating growth and survival.Cytotechnology · 2007Article
- Control and regulation of the cellular responses to cold shock: the responses in yeast and mammalian systems.The Biochemical journal · 2006Review
- Enhancement of productivity of recombinant alpha-amidating enzyme by low temperature culture.Cytotechnology · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to seek an efficient method for producing a recombinant protein by using animal cell culture, we investigated various effects of the culture temperature on a recombinant CHO cell line (3µ-1S), producing a C-terminal α-amidating enzyme (799BglIIα-AE) originating from Xenopus laevis. The results revealed that a low culture temperature (below 37 °C) led to the following phenomena: [1] inhibited cell growth, [2] enhanced cellular productivity of the recombinant protein, [3] maintained high cell viability, [4] suppressed medium consumption, and [5] suppressed release of impurities from the cells. These findings indicate that a quite simple method, the culture at low temperature, will contribute to the total improvement of the industrial process for the production of the recombinant protein, 799BglIIα-AE.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.