Evidence map›Paper›PMID 22358233›Full record

ArticleClinics (Sao Paulo, Brazil)2012

Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation.

Adila A Hamid, Ruszymah Bt Hj Idrus, Aminuddin Bin Saim, Somasumdaram Sathappan, Kien-Hui Chua

Abstract read
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In one paragraph

Article in Clinics (Sao Paulo, Brazil), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 2 syntheses or guidelines pooled it.

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  18. Synergistic interaction of hTGF-βBMC biotechnology · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Adila A HamidUniversiti Kebangsaan Malaysia, Department of Physiology, Faculty of Medicine, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.
Ruszymah Bt Hj Idrus
Aminuddin Bin Saim
Somasumdaram Sathappan
Kien-Hui Chua

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesUnderstanding the changes in chondrogenic gene expression that are involved in the differentiation of human adipose-derived stem cells to chondrogenic cells is important prior to using this approach for cartilage repair. The aims of the study were to characterize human adipose-derived stem cells and to examine chondrogenic gene expression after one, two, and three weeks of induction. MATERIALS AND

methodsHuman adipose-derived stem cells at passage 4 were evaluated by flow cytometry to examine the expression of surface markers. These adipose-derived stem cells were tested for adipogenic and osteogenic differentiation capacity. Ribonucleic acid was extracted from the cells for quantitative polymerase chain reaction analysis to determine the expression levels of chondrogenic genes after chondrogenic induction.

resultsHuman adipose-derived stem cells were strongly positive for the mesenchymal markers CD90, CD73, CD44, CD9, and histocompatibility antigen and successfully differentiated into adipogenic and osteogenic lineages. The human adipose-derived stem cells aggregated and formed a dense matrix after chondrogenic induction. The expression of chondrogenic genes (collagen type II, aggrecan core protein, collagen type XI, COMP, and ELASTIN) was significantly higher after the first week of induction. However, a significantly elevated expression of collagen type X was observed after three weeks of chondrogenic induction.

conclusionHuman adipose-derived stem cells retain stem cell characteristics after expansion in culture to passage 4 and serve as a feasible source of cells for cartilage regeneration. Chondrogenesis in human adipose-derived stem cells was most prominent after one week of chondrogenic induction.

Indexed as

AdipogenesisAdipose TissueBiomarkersCartilage, ArticularCell DifferentiationCells, CulturedChondrocytesChondrogenesisCollagenElastinFlow CytometryGene Expression RegulationHumansMesenchymal Stem CellsOsteogenesisRNA, MessengerBiomarkersCollagenElastinRNA, MessengerSOX9 Transcription Factor

Identifiers

PMID22358233
PMCPMC3275119

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.