ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012
Phospholipase C-mediated suppression of dark noise enables single-photon detection in Drosophila photoreceptors.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 27 citations in OpenAlex.
- Retinal TRP channels: Cell-type-specific regulators of retinal homeostasis and multimodal integration.Progress in retinal and eye research · 2023Review
- The Role of Membrane Lipids in Light-Activation ofBiomolecules · 2022Review
- Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors.Journal of visualized experiments : JoVE · 2017Article
- The Phosphorylation State of theThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- The remarkable visual capacities of nocturnal insects: vision at the limits with small eyes and tiny brains.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Ectopic Expression of Mouse Melanopsin inThe Journal of biological chemistry · 2017Article
- Biophotons Contribute to Retinal Dark Noise.Neuroscience bulletin · 2016Article
- Functional cooperation between the IP3 receptor and phospholipase C secures the high sensitivity to light of Drosophila photoreceptors in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Speed and sensitivity of phototransduction in Drosophila depend on degree of saturation of membrane phospholipids.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Drosophila TRP and TRPL are assembled as homomultimeric channels in vivo.Journal of cell science · 2013Article
- Common mechanisms regulating dark noise and quantum bump amplification in Drosophila photoreceptors.Journal of neurophysiology · 2013Article
- Compartmentalization and Ca2+ buffering are essential for prevention of light-induced retinal degeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Phototransduction ineNeuroArticle
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Drosophila photoreceptor cells use the ubiquitous G-protein-mediated phospholipase C (PLC) cascade to achieve ultimate single-photon sensitivity. This is manifested in the single-photon responses (quantum bumps). In photoreceptor cells, dark activation of G(q)α molecules occurs spontaneously and produces unitary dark events (dark bumps). A high rate of spontaneous G(q)α activation and dark bump production potentially hampers single-photon detection. We found that in wild-type flies the in vivo rate of spontaneous G(q)α activation is very high. Nevertheless, this high rate is not manifested in a substantially high rate of dark bumps. Therefore, it is unclear how phototransduction suppresses dark bump production arising from spontaneous G(q)α activation, while still maintaining high-fidelity representation of single photons. In this study we show that reduced PLC catalytic activity selectively suppressed production of dark bumps but not light-induced bumps. Manipulations of PLC activity using PLC mutant flies and Ca(2+) modulations revealed that a critical level of PLC activity is required to induce bump production. The required minimal level of PLC activity selectively suppressed random production of single G(q)α-activated dark bumps despite a high rate of spontaneous G(q)α activation. This minimal PLC activity level is reliably obtained by photon-induced synchronized activation of several neighboring G(q)α molecules activating several PLC molecules, but not by random activation of single G(q)α molecules. We thus demonstrate how a G-protein-mediated transduction system, with PLC as its target, selectively suppresses its intrinsic noise while preserving reliable signaling.
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