ArticleMolecular biology of the cell2012
Requirement of translocated lysosomal V1 H(+)-ATPase for activation of membrane acid sphingomyelinase and raft clustering in coronary endothelial cells.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
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- MicroRNA-10a enrichment in factor VIIa-released endothelial extracellular vesicles: potential mechanisms.Journal of thrombosis and haemostasis : JTH · 2024Article
- A Comprehensive Review on the Interplay betweenCells · 2021Review
- Lysosomal TRPML1 Channel: Implications in Cardiovascular and Kidney Diseases.Advances in experimental medicine and biology · 2021Article
- Article
- Acid sphingomyelinase downregulation alleviates vascular endothelial leptin resistance in rats.Acta pharmacologica Sinica · 2020Article
- Fusion of lysosomes to plasma membrane initiates radiation-induced apoptosis.The Journal of cell biology · 2020Article
- Review
- Membrane rafts-redox signalling pathway contributes to renal fibrosis via modulation of the renal tubular epithelial-mesenchymal transition.The Journal of physiology · 2018Article
- Electron Paramagnetic Resonance Measurements of Reactive Oxygen Species by Cyclic Hydroxylamine Spin Probes.Antioxidants & redox signaling · 2018Review
- Sphingosine rescues aged mice from pulmonary pseudomonas infection.The Journal of surgical research · 2017Article
- Plasma Membrane Repair Is Regulated Extracellularly by Proteases Released from Lysosomes.PloS one · 2016Article
- Lipid raft-dependent plasma membrane repair interferes with the activation of B lymphocytes.The Journal of cell biology · 2015Article
- Regulation of hematogenous tumor metastasis by acid sphingomyelinase.EMBO molecular medicine · 2015Article
- Acid sphingomyelinase inhibition protects mice from lung edema and lethal Staphylococcus aureus sepsis.Journal of molecular medicine (Berlin, Germany) · 2015Article
- Damage control: cellular mechanisms of plasma membrane repair.Trends in cell biology · 2014Review
- Sphingoid long chain bases prevent lung infection by Pseudomonas aeruginosa.EMBO molecular medicine · 2014Article
- Regulation of autophagic flux by dynein-mediated autophagosomes trafficking in mouse coronary arterial myocytes.Biochimica et biophysica acta · 2013Article
- Membrane signaling induced by high doses of ionizing radiation in the endothelial compartment. Relevance in radiation toxicity.International journal of molecular sciences · 2013Review
- Fluid shear stress induces the clustering of heparan sulfate via mobility of glypican-1 in lipid rafts.American journal of physiology. Heart and circulatory physiology · 2013Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Acid sphingomyelinase (ASM) mediates the formation of membrane raft (MR) redox signalosomes in a process that depends on a local acid microenvironment in coronary arterial endothelial cells (CAECs). However, it is not known how this local acid microenvironment is formed and maintained. The present study hypothesized that lysosomal V1 H(+)-ATPase provides a hospitable acid microenvironment for activation of ASM when lysosomes traffic and fuse into the cell membrane. Confocal microscopy showed that local pH change significantly affected MRs, with more fluorescent patches under low pH. Correspondingly, the ASM product, ceramide, increased locally in the cell membrane. Electron spin resonance assay showed that local pH increase significantly inhibited NADPH oxidase-mediated production of O(2)(-.) in CAECs. Direct confocal microscopy demonstrated that Fas ligand resulted in localized areas of decreased pH around CAEC membranes. The inhibitors of both lysosomal fusion and H(+)-ATPase apparently attenuated FasL-caused pH decrease. V1 H(+)-ATPase accumulation and activity on cell membranes were substantially suppressed by the inhibitors of lysosomal fusion or H(+)-ATPase. These results provide the first direct evidence that translocated lysosomal V1 H(+)-ATPase critically contributes to the formation of local acid microenvironment to facilitate activation of ASM and consequent MR aggregation, forming MR redox signalosomes and mediating redox signaling in CAECs.
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