ArticleJournal of biosciences2012
Development of transgenic finger millet (Eleusine coracana (L.) Gaertn.) resistant to leaf blast disease.
Article in Journal of biosciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 104 citations in OpenAlex.
- Rapid Agrobacterium-mediated transformation and high-efficiency regeneration of finger millet (Eleusine coracana) for crop improvement.Transgenic research · 2026Article
- Integrative insights into abiotic stress tolerance in finger millet (Frontiers in plant science · 2026Review
- Genome-Wide Analysis Reveals Chitinases as Putative Defense-Related Proteins Against Fungi in the Genomes ofPlants (Basel, Switzerland) · 2025Article
- Enhancing tiny millets through genome editing: current status and future prospects.Molecular genetics and genomics : MGG · 2025Review
- Review
- Biotechnological studies towards improvement of finger millet using multi-omics approaches.Functional & integrative genomics · 2024Review
- Review
- Co-overexpression of chitinase and β-1,3-glucanase significantly enhanced the resistance of Iranian wheat cultivars to Fusarium.BMC biotechnology · 2024Article
- Characterization of soybean chitinase genes induced by rhizobacteria involved in the defense againstFrontiers in plant science · 2024Article
- Genetic engineering and genome editing technologies as catalyst for Africa's food security: the case of plant biotechnology in Nigeria.Frontiers in genome editing · 2024Review
- Review
- Wheat blast: The last enemy of hunger fighters.Genetics and molecular biology · 2023Article
- Genome-editing in millets: current knowledge and future perspectives.Molecular biology reports · 2022Review
- Silicon supplementation improves early blight resistance in3 Biotech · 2021Article
- A Review of Recent Advances and Future Directions in the Management of Salinity Stress in Finger Millet.Frontiers in plant science · 2021Review
- Integration of transcriptomic and proteomic analyses for finger millet [Eleusine coracana (L.) Gaertn.] in response to drought stress.PloS one · 2021Article
- Breeding Strategies and Challenges in the Improvement of Blast Disease Resistance in Finger Millet. A Current Review.Frontiers in plant science · 2020Review
- Review
- Association between sheath blight resistance and chitinase activity in transgenic rice plants expressing McCHIT1 from bitter melon.Transgenic research · 2019Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Finger millet plants conferring resistance to leaf blast disease have been developed by inserting a rice chitinase (chi11) gene through Agrobacterium-mediated transformation. Plasmid pHyg-Chi.11 harbouring the rice chitinase gene under the control of maize ubiquitin promoter was introduced into finger millet using Agrobacterium strain LBA4404 (pSB1). Transformed plants were selected and regenerated on hygromycin-supplemented medium. Transient expression of transgene was confirmed by GUS histochemical staining. The incorporation of rice chitinase gene in R0 and R1 progenies was confirmed by PCR and Southern blot analyses. Expression of chitinase gene in finger millet was confirmed by Western blot analysis with a barley chitinase antibody. A leaf blast assay was also performed by challenging the transgenic plants with spores of Pyricularia grisea. The frequency of transient expression was 16.3% to 19.3%. Stable frequency was 3.5% to 3.9%. Southern blot analysis confirmed the integration of 3.1 kb chitinase gene. Western blot analysis detected the presence of 35 kDa chitinase enzyme. Chitinase activity ranged from 19.4 to 24.8. In segregation analysis, the transgenic R1 lines produced three resistant and one sensitive for hygromycin, confirming the normal Mendelian pattern of transgene segregation. Transgenic plants showed high level of resistance to leaf blast disease compared to control plants. This is the first study reporting the introduction of rice chitinase gene into finger millet for leaf blast resistance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.