ArticleJournal of biosciences2012
Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants.
Article in Journal of biosciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- Plant Viral Vectors for Vaccine Development.Vaccines · 2026Review
- Review
- A GoldenBraid-Compatible Virus-Based Vector System for Transient Expression of Heterologous Proteins in Plants.Viruses · 2022Article
- Immunity functions of Arabidopsis pathogenesis-related 1 are coupled but not confined to its C-terminus processing and trafficking.Molecular plant pathology · 2022Article
- Contributions of the international plant science community to the fight against human infectious diseases - part 1: epidemic and pandemic diseases.Plant biotechnology journal · 2021Review
- Atomic structure of potato virus X, the prototype of the Alphaflexiviridae family.Nature chemical biology · 2020Article
- Production and characterization of virus-like particles of grapevine fanleaf virus presenting L2 epitope of human papillomavirus minor capsid protein.BMC biotechnology · 2019Article
- Small, Smaller, Nano: New Applications for Potato Virus X in Nanotechnology.Frontiers in plant science · 2019Review
- Computational and biological characterization of fusion proteins of two insecticidal proteins for control of insect pests.Scientific reports · 2018Article
- Advances in Designing and Developing Vaccines, Drugs and Therapeutic Approaches to Counter Human Papilloma Virus.Frontiers in immunology · 2018Review
- Recombinant Plant Engineering for Immunotherapeutic Production.Current molecular biology reports · 2017Review
- Plant-based vaccines for animals and humans: recent advances in technology and clinical trials.Therapeutic advances in vaccines · 2015Review
- Recent developments in therapeutic protein expression technologies in plants.Biotechnology letters · 2015Review
- Current status of viral expression systems in plants and perspectives for oral vaccines development.Plant molecular biology · 2015Review
- The Two-Faced Potato Virus X: From Plant Pathogen to Smart Nanoparticle.Frontiers in plant science · 2015Review
- Plant-based vaccines against viruses.Virology journal · 2014Review
- Recent advances on host plants and expression cassettes' structure and function in plant molecular pharming.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2014Review
- Second-generation prophylactic HPV vaccines: successes and challenges.Expert review of vaccines · 2014Review
- Plant virus expression vector development: new perspectives.BioMed research international · 2014Review
- N-glycosylation modification of plant-derived virus-like particles: an application in vaccines.BioMed research international · 2014Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transient expression of foreign genes based on plant viral vectors is a suitable system for the production of relevant immunogens that can be used for the development of a new generation of vaccines against a variety of infectious diseases. In the present study the epitope derived from HPV-16 L2 minor capsid protein (amino acids 108-120) was expressed from Potato virus X (PVX)-based vector pGR106 as N- or C-terminal fusion with the PVX coat protein (PVX CP) in transgenic Nicotiana benthamiana plants. The fusion protein L2 108-120-PVX CP was successfully expressed in plants at a level of 170 mg/kg of fresh leaf tissue. The C-terminal fusion protein PVX CP- L2 108-120 was expressed using mutated vector sequence to avoid homologous recombination at a level of 8 mg/kg of fresh leaf tissue. Immunogenicity of L2 108-120-PVX CP virus-like particles was tested after immunization of mice by subcutaneous injection or tattoo administration. In animal sera the antibodies against the PVX CP and the L2 108-120 epitope were found after both methods of vaccine delivery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.