ArticleJournal of neuroinflammation2012
Interaction of HmC1q with leech microglial cells: involvement of C1qBP-related molecule in the induction of cell chemotaxis.
Article in Journal of neuroinflammation, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Article
- Evolution of Microglia.Advances in neurobiology · 2024Article
- Invertebrate C1q Domain-Containing Proteins: Molecular Structure, Functional Properties and Biomedical Potential.Marine drugs · 2023Review
- Neuroprotective versus Neuroinflammatory Roles of Complement: From Development to Disease.Trends in neurosciences · 2021Review
- A Comparative Biology of Microglia Across Species.Frontiers in cell and developmental biology · 2021Review
- Isolation of microglia-derived extracellular vesicles: towards miRNA signatures and neuroprotection.Journal of nanobiotechnology · 2019Article
- ALK4/5-dependent TGF-β signaling contributes to the crosstalk between neurons and microglia following axonal lesion.Scientific reports · 2019Article
- Medicinal Leech CNS as a Model for Exosome Studies in the Crosstalk between Microglia and Neurons.International journal of molecular sciences · 2018Article
- C1QBP is upregulated in colon cancer and binds to apolipoprotein A-I.Experimental and therapeutic medicine · 2017Article
- Calreticulin contributes to C1q-dependent recruitment of microglia in the leech Hirudo medicinalis following a CNS injury.Medical science monitor : international medical journal of experimental and clinical research · 2014Article
- The leech nervous system: a valuable model to study the microglia involvement in regenerative processes.Clinical & developmental immunology · 2013Review
- Report on the 12th symposium on invertebrate neurobiology held 31 August-4 September 2011 at the Balaton Limnological Research Institute of the Hungarian Academy of Sciences, Tihany, Hungary.Invertebrate neuroscience : IN · 2012Article
- Article
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10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn invertebrates, the medicinal leech is considered to be an interesting and appropriate model to study neuroimmune mechanisms. Indeed, this non-vertebrate animal can restore normal function of its central nervous system (CNS) after injury. Microglia accumulation at the damage site has been shown to be required for axon sprouting and for efficient regeneration. We characterized HmC1q as a novel chemotactic factor for leech microglial cell recruitment. In mammals, a C1q-binding protein (C1qBP alias gC1qR), which interacts with the globular head of C1q, has been reported to participate in C1q-mediated chemotaxis of blood immune cells. In this study, we evaluated the chemotactic activities of a recombinant form of HmC1q and its interaction with a newly characterized leech C1qBP that acts as its potential ligand.
methodsRecombinant HmC1q (rHmC1q) was produced in the yeast Pichia pastoris. Chemotaxis assays were performed to investigate rHmC1q-dependent microglia migration. The involvement of a C1qBP-related molecule in this chemotaxis mechanism was assessed by flow cytometry and with affinity purification experiments. The cellular localization of C1qBP mRNA and protein in leech was investigated using immunohistochemistry and in situ hybridization techniques.
resultsrHmC1q-stimulated microglia migrate in a dose-dependent manner. This rHmC1q-induced chemotaxis was reduced when cells were preincubated with either anti-HmC1q or anti-human C1qBP antibodies. A C1qBP-related molecule was characterized in leech microglia.
conclusionsA previous study showed that recruitment of microglia is observed after HmC1q release at the cut end of axons. Here, we demonstrate that rHmC1q-dependent chemotaxis might be driven via a HmC1q-binding protein located on the microglial cell surface. Taken together, these results highlight the importance of the interaction between C1q and C1qBP in microglial activation leading to nerve repair in the medicinal leech.
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