ArticleMolecular microbiology2012
Targeting of csgD by the small regulatory RNA RprA links stationary phase, biofilm formation and cell envelope stress in Escherichia coli.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed.
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- From environmental signals to adaptive phenotypes: signal-responsive regulation and network logic of bacterial small RNAs.FEMS microbiology reviews · 2026Review
- Characterization of Antibiofilm Molecules from Bovine Coagulase-Negative Staphylococci.PloS one · 2026Article
- Effects of multiple cell regulators on curli gene expression inJournal of bacteriology · 2025Article
- RcsB and H-NS Both Contribute to the Repression the Expression of theMicroorganisms · 2025Article
- The RNA Chaperone Hfq and Small Non-Coding RNAs Modulate the Biofilm Formation of the Fish PathogenInternational journal of molecular sciences · 2025Article
- Control of iron acquisition by multiple small RNAs unravels a new role for transcriptional terminator loops in gene regulation.Nucleic acids research · 2024Article
- A phosphate starvation induced small RNA promotes Bacillus biofilm formation.NPJ biofilms and microbiomes · 2024Article
- Fluoropyrimidines affectBiofilm · 2024Article
- The role of RNA regulators, quorum sensing and c-di-GMP in bacterial biofilm formation.FEBS open bio · 2023Review
- Natural Strategies as Potential Weapons against Bacterial Biofilms.Life (Basel, Switzerland) · 2022Review
- Role of Hfq in glucose utilization, biofilm formation and quorum sensing system in Bacillus subtilis.Biotechnology letters · 2022Article
- What Is in a Cat Scratch? Growth ofMicroorganisms · 2021Review
- A genetic screen to identify factors affected by undecaprenyl phosphate recycling uncovers novel connections to morphogenesis in Escherichia coli.Molecular microbiology · 2021Article
- Orchestration of virulence factor expression and modulation of biofilm dispersal in Erwinia amylovora through activation of the Hfq-dependent small RNA RprA.Molecular plant pathology · 2021Article
- Transcriptional and Post-transcriptional Control of the Nitrate Respiration in Bacteria.Frontiers in molecular biosciences · 2021Review
- Local c-di-GMP Signaling in the Control of Synthesis of the E. coli Biofilm Exopolysaccharide pEtN-Cellulose.Journal of molecular biology · 2020Article
- Small RNAs OmrA and OmrB promote class III flagellar gene expression by inhibiting the synthesis of anti-Sigma factor FlgM.RNA biology · 2020Article
- Trans-Acting Small RNAs and Their Effects on Gene Expression inEcoSal Plus · 2020Review
- Mechanism for coordinate regulation ofRNA biology · 2020Article
19 more citing papers are in PubMed but not listed here.
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8 authors.
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Abstract
RprA is a small regulatory RNA known to weakly affect the translation of σ(S) (RpoS) in Escherichia coli. Here we demonstrate that csgD, which encodes a stationary phase-induced biofilm regulator, as well as ydaM, which encodes a diguanylate cyclase involved in activating csgD transcription, are novel negatively controlled RprA targets. As shown by extensive mutational analysis, direct binding of RprA to the 5'-untranslated and translational initiation regions of csgD mRNA inhibits translation and reduces csgD mRNA levels. In the case of ydaM mRNA, RprA base-pairs directly downstream of the translational start codon. In a feedforward loop, RprA can thus downregulate > 30 YdaM/CsgD-activated genes including those for adhesive curli fimbriae. However, during early stationary phase, when csgD transcription is strongly activated, the synthesis of csgD mRNA exceeds that of RprA, which allows the accumulation of CsgD protein. This situation is reversed when csgD transcription is shut off - for instance, later in stationary phase or during biofilm formation - or by conditions that further activate RprA expression via the Rcs two-component system. Thus, antagonistic regulation of csgD and RprA at the mRNA level integrates cell envelope stress signals with global gene expression during stationary phase and biofilm formation.
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