ArticleTissue engineering. Part C, Methods2012
Rapid generation of multiplexed cell cocultures using acoustic droplet ejection followed by aqueous two-phase exclusion patterning.
Article in Tissue engineering. Part C, Methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Acoustic Bioprinting: A Glimpse Into an Emerging Field.Small methods · 2026Review
- Printing of Cantilevers and Millifluidic Devices Using Ultrasound Waves.3D printing and additive manufacturing · 2025Article
- Integrating large language model-based agents into a virtual patient chatbot for clinical anamnesis training.Computational and structural biotechnology journal · 2025Article
- Bioprinted research models of urological malignancy.Exploration (Beijing, China) · 2024Review
- The Evolution of Technology-Driven In Vitro Models for Neurodegenerative Diseases.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Research Progress in the Field of Tumor Model Construction Using Bioprinting: A Review.International journal of nanomedicine · 2024Review
- Crossover of surface waves and capillary-viscous-elastic transition in soft biomaterials detected by resonant acoustic rheometry.Biomaterials · 2023Article
- A Comprehensive Review of Surface Acoustic Wave-Enabled Acoustic Droplet Ejection Technology and Its Applications.Micromachines · 2023Review
- Advances in Single-Cell Printing.Micromachines · 2022Review
- 3D Bioprinting for Spinal Cord Injury Repair.Frontiers in bioengineering and biotechnology · 2022Review
- Bioink Formulations for Bone Tissue Regeneration.Frontiers in bioengineering and biotechnology · 2021Review
- Emulating Human Tissues and Organs: A Bioprinting Perspective Toward Personalized Medicine.Chemical reviews · 2020Review
- Acoustic Patterning of Growth Factor for Three-Dimensional Tissue Engineering.Tissue engineering. Part A · 2020Article
- Three-Dimensional Printing and Cell Therapy for Wound Repair.Advances in wound care · 2018Review
- The potential of 3D printing in urological research and patient care.Nature reviews. Urology · 2018Review
- 3D bioprinting processes: A perspective on classification and terminology.International journal of bioprinting · 2018Article
- Freeform micropatterning of living cells into cell culture medium using direct inkjet printing.Scientific reports · 2017Article
- The Application of Ultrasound in 3D Bio-Printing.Molecules (Basel, Switzerland) · 2016Review
- Review
- Recent developments in multiplexing techniques for immunohistochemistry.Expert review of molecular diagnostics · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
The development of tools for patterning cocultures of cells is a fundamental interest among cell biologists and tissue engineers. Although a variety of systems exist for micropatterning cells, the methods used to generate cell micropatterns are often cumbersome and difficult to adapt for tissue engineering purposes. This study combines acoustic droplet ejection and aqueous two-phase system exclusion patterning to introduce a method for patterning cocultures of cells in multiplexed arrays. This new method uses focused acoustic radiation pressure to eject discrete droplets of uniform size from the surface of a dextran solution containing cells. The size of droplets is controlled by adjusting ultrasound parameters, such as pulse, duration, and amplitude. The ejected dextran droplets are captured on a cell culture substrate that is manipulated by a computer-controlled 3D positioning system according to predesigned patterns. Polyethylene glycol solution containing an additional cell type is then added to the culture dish to produce a two-phase system capable of depositing different types of cells around the initial pattern of cells. We demonstrate that our method can produce patterns of islands or lines with two or more cell types. Further, we demonstrate that patterns can be multiplexed for studies involving combinations of multiple cell types. This method offers a tool to transfer cell-containing samples in a contact-free, nozzle-less manner, avoiding sample cross-contamination. It can be used to pattern cell cocultures without complicated fabrication of culture substrates. These capabilities were used to examine the response of cancer cells to the presence of a ligand (CXCL12) secreted from surrounding cocultured cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.