ArticleJournal of microscopy2012
Quantitative comparison between full-spectrum and filter-based imaging in hyperspectral fluorescence microscopy.
Article in Journal of microscopy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed, 11 citations in OpenAlex.
- Multifaceted mirror array illuminator for fluorescence excitation-scanning spectral imaging microscopy.Journal of biomedical optics · 2023Article
- A review of snapshot multidimensional optical imaging: measuring photon tags in parallel.Physics reports · 2016Article
- Hyperspectral Image Mapping Spectrometry for Retinal Oximetry Measurements in Four Diseased Eyes.International ophthalmology clinics · 2016Article
- Photoacoustic microscopy and computed tomography: from bench to bedside.Annual review of biomedical engineering · 2014Review
- Excitation-scanning hyperspectral imaging microscope.Journal of biomedical optics · 2014Article
- High-speed fluorescence excitation-scanning hyperspectral imaging microscopy using thin-film tunable filters.Proceedings of SPIE--the International Society for Optical EngineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We implement a filterless illumination scheme on a hyperspectral fluorescence microscope to achieve full-range spectral imaging. The microscope employs polarisation filtering, spatial filtering and spectral unmixing filtering to replace the role of traditional filters. Quantitative comparisons between full-spectrum and filter-based microscopy are provided in the context of signal dynamic range and accuracy of measured fluorophores' emission spectra. To show potential applications, a five-colour cell immunofluorescence imaging experiment is theoretically simulated. Simulation results indicate that the use of proposed full-spectrum imaging technique may result in three times improvement in signal dynamic range compared to that can be achieved in the filter-based imaging.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.