ArticleScientific reports2011
Application and comparison of large-scale solution-based DNA capture-enrichment methods on ancient DNA.
Article in Scientific reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Exploring the Quality of Historic DNA From Pinned Beetles.Ecology and evolution · 2026Article
- A Roadmap for Using Hybridisation Capture-Based Target Enrichment of Ancient Environmental DNA in Palaeoecology.Molecular ecology resources · 2026Review
- Optimizing ancient DNA recovery from archaeological plant seeds.Scientific reports · 2025Article
- Article
- Review: Computational analysis of human skeletal remains in ancient DNA and forensic genetics.iScience · 2023Review
- Low hybridization temperatures improve target capture success of invertebrate loci: a case study of leaf-footed bugs (Hemiptera: Coreoidea).Royal Society open science · 2023Article
- Fishing for DNA? Designing baits for population genetics in target enrichment experiments: Guidelines, considerations and the new tool supeRbaits.Molecular ecology resources · 2022Article
- Holocene life and microbiome profiling in ancient tropical Lake Chalco, Mexico.Scientific reports · 2021Article
- Quantitative Human Paleogenetics: What can Ancient DNA Tell us About Complex Trait Evolution?Frontiers in genetics · 2021Review
- Ancient pathogen genomics as an emerging tool for infectious disease research.Nature reviews. Genetics · 2019Review
- The efficacy of whole human genome capture on ancient dental calculus and dentin.American journal of physical anthropology · 2019Article
- In-solution Y-chromosome capture-enrichment on ancient DNA libraries.BMC genomics · 2018Article
- Article
- A Robust Framework for Microbial Archaeology.Annual review of genomics and human genetics · 2017Review
- Comprehensive analysis of microorganisms accompanying human archaeological remains.GigaScience · 2017Article
- Zooplankton Community Profiling in a Eutrophic Freshwater Ecosystem-Lake Tai Basin by DNA Metabarcoding.Scientific reports · 2017Article
- Human evolution: a tale from ancient genomes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Improving ancient DNA genome assembly.PeerJ · 2017Article
- The efficacy of high-throughput sequencing and target enrichment on charred archaeobotanical remains.Scientific reports · 2016Article
- Barcoding the largest animals on Earth: ongoing challenges and molecular solutions in the taxonomic identification of ancient cetaceans.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
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11 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The development of second-generation sequencing technologies has greatly benefitted the field of ancient DNA (aDNA). Its application can be further exploited by the use of targeted capture-enrichment methods to overcome restrictions posed by low endogenous and contaminating DNA in ancient samples. We tested the performance of Agilent's SureSelect and Mycroarray's MySelect in-solution capture systems on Illumina sequencing libraries built from ancient maize to identify key factors influencing aDNA capture experiments. High levels of clonality as well as the presence of multiple-copy sequences in the capture targets led to biases in the data regardless of the capture method. Neither method consistently outperformed the other in terms of average target enrichment, and no obvious difference was observed either when two tiling designs were compared. In addition to demonstrating the plausibility of capturing aDNA from ancient plant material, our results also enable us to provide useful recommendations for those planning targeted-sequencing on aDNA.
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