Evidence map›Paper›PMID 22355544›Full record

ArticleScientific reports2011

Time-course analysis of the Shewanella amazonensis SB2B proteome in response to sodium chloride shock.

J Jacob Parnell, Stephen J Callister, Giovanni Rompato, Carrie D Nicora, Ljiljana Paša-Tolić, Ashley Williamson, Michael E Pfrender

Open access · goldAbstract readValidation Study
In one paragraph

Article in Scientific reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

J Jacob ParnellCenter for Integrated BioSystems and Department of Biology, Utah State University, Logan, UT, USA. jacob.parnell@usu.edu
Stephen J Callister
Giovanni Rompato
Carrie D Nicora
Ljiljana Paša-Tolić
Ashley Williamson
Michael E Pfrender
Utah State University · USLogan Regional Hospital · USPacific Northwest National Laboratory · USUniversity of Notre Dame · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Shewanellae are microbial models for environmental stress response; however, the sequential expression of mechanisms in response to stress is poorly understood. Here we experimentally determine the response mechanisms of Shewanella amazonensis SB2B during sodium chloride stress using a novel liquid chromatography and accurate mass-time tag mass spectrometry time-course proteomics approach. The response of SB2B involves an orchestrated sequence of events comprising increased signal transduction associated with motility and restricted growth. Following a metabolic shift to branched chain amino acid degradation, motility and cellular replication proteins return to pre-perturbed levels. Although sodium chloride stress is associated with a change in the membrane fatty acid composition in other organisms, this is not the case for SB2B as fatty acid degradation pathways are not expressed and no change in the fatty acid profile is observed. These findings suggest that shifts in membrane composition may be an indirect physiological response to high NaCl stress.

Indexed as

ProteomeBacterial ProteinsBase SequenceChromatography, LiquidDNA PrimersKineticsMass SpectrometryPolymerase Chain ReactionShewanellaSodium ChlorideBacterial ProteinsDNA PrimersProteomeSodium Chloride

Identifiers

PMID22355544
PMCPMC3216512
OpenAlexW2152929221

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.