ArticleScientific reports2011
Halophilic enzyme activation induced by salts.
Article in Scientific reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 76 citations in OpenAlex.
- Polyhydroxyalkanoate synthesis ability of the salt-tolerant bacterial strain Salinicola lusitanus BTNC26 isolated from the coastal zone in Vietnam and its application in food packaging film production.World journal of microbiology & biotechnology · 2026Article
- Investigation of salt-bridge interactions underlying salt activation of halophilic methionine sulfoxide reductase A.Frontiers in microbiology · 2026Article
- Xylan Degradation in the Halotolerant BacteriumJournal of agricultural and food chemistry · 2025Article
- How sensitive are protein hydration shells to electrolyte concentration and protein composition?Protein science : a publication of the Protein Society · 2025Article
- HaloClass: Salt-Tolerant Protein Classification with Protein Language Models.The protein journal · 2024Article
- Electrostatics introduce a trade-off between mesophilic stability and adaptation in halophilic proteins.Protein science : a publication of the Protein Society · 2024Article
- Microbial-derived salt-tolerant proteases and their applications in high-salt traditional soybean fermented foods: a review.Bioresources and bioprocessing · 2023Review
- Characterization of a novel cold-adapted intracellular serine protease from the extremophileFrontiers in microbiology · 2023Article
- Microbiological Safety and Shelf-Life of Low-Salt Meat Products-A Review.Foods (Basel, Switzerland) · 2022Review
- Biophysical and structural studies reveal marginal stability of a crucial hydrocarbon biosynthetic enzyme acyl ACP reductase.Scientific reports · 2021Article
- Molecular cloning and characterization of a thermostable and halotolerant endo-β-1,4-glucanase from3 Biotech · 2021Article
- Article
- Biosynthesis of butyl esters from crude oil of palm fruit and kernel using halophilic lipase secretion by3 Biotech · 2019Article
- A polyextremophilic alcohol dehydrogenase from the Atlantis II Deep Red Sea brine pool.FEBS open bio · 2019Article
- Mechanism of salt-induced activity enhancement of a marine-derived laccase, Lac15.European biophysics journal : EBJ · 2018Article
- Building a Geochemical View of Microbial Salt Tolerance: Halophilic Adaptation ofFrontiers in microbiology · 2018Article
- Halophiles and their enzymes: negativity put to good use.Current opinion in microbiology · 2015Review
- Effects of salt on the structure, stability, and function of a halophilic dihydrofolate reductase from a hyperhalophilic archaeon, Haloarcula japonica strain TR-1.Extremophiles : life under extreme conditions · 2015Article
- Archaeal Nucleic Acid Ligases and Their Potential in Biotechnology.Archaea (Vancouver, B.C.) · 2015Review
- Coexistence of bacterial leucyl-tRNA synthetases with archaeal tRNA binding domains that distinguish tRNA(Leu) in the archaeal mode.Nucleic acids research · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Halophilic archea (halobacteriae) thrive in hypersaline environments, avoiding osmotic shock by increasing the ion concentration of their cytoplasm by up to 3-6 M. To remain folded and active, their constitutive proteins have evolved towards a biased amino acid composition. High salt concentration affects catalytic activity in an enzyme-dependent way and a unified molecular mechanism remains elusive. Here, we have investigated a DNA ligase from Haloferax volcanii (Hv LigN) to show that K(+) triggers catalytic activity by preferentially stabilising a specific conformation in the reaction coordinate. Sodium ions, in turn, do not populate such isoform and the enzyme remains inactive in the presence of this co-solute. Our results show that the halophilic amino acid signature enhances the enzyme's thermodynamic stability, with an indirect effect on its catalytic activity. This model has been successfully applied to reengineer Hv LigN into an enzyme that is catalytically active in the presence of NaCl.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.