ArticlePloS one2012
Development and comparison of two assay formats for parallel detection of four biothreat pathogens by using suspension microarrays.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Development of multiplexed bead arrays for the simultaneous detection of nucleic acid from multiple viruses in bat samples.Journal of virological methods · 2015Article
- DNA microarray-based detection of Coxiella burnetii, the causative agent of Q fever.Acta veterinaria Scandinavica · 2014Article
- Influence of the length of target DNA overhang proximal to the array surface on discrimination of single-base mismatches on a 25-mer oligonucleotide array.BMC research notes · 2014Article
- Coxiella burnetii - Pathogenic Agent of Q (Query) Fever.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2014Article
- A system to simultaneously detect tick-borne pathogens based on the variability of the 16S ribosomal genes.Parasites & vectors · 2013Article
- Multiplex qPCR for reliable detection and differentiation of Burkholderia mallei and Burkholderia pseudomallei.BMC infectious diseases · 2013Article
- Simultaneous quantification of multiple bacteria by the BactoChip microarray designed to target species-specific marker genes.PloS one · 2013Article
- Microsphere suspension array assays for detection and differentiation of Hendra and Nipah viruses.BioMed research international · 2013Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microarrays provide a powerful analytical tool for the simultaneous detection of multiple pathogens. We developed diagnostic suspension microarrays for sensitive and specific detection of the biothreat pathogens Bacillus anthracis, Yersinia pestis, Francisella tularensis and Coxiella burnetii. Two assay chemistries for amplification and labeling were developed, one method using direct hybridization and the other using target-specific primer extension, combined with hybridization to universal arrays. Asymmetric PCR products for both assay chemistries were produced by using a multiplex asymmetric PCR amplifying 16 DNA signatures (16-plex). The performances of both assay chemistries were compared and their advantages and disadvantages are discussed. The developed microarrays detected multiple signature sequences and an internal control which made it possible to confidently identify the targeted pathogens and assess their virulence potential. The microarrays were highly specific and detected various strains of the targeted pathogens. Detection limits for the different pathogen signatures were similar or slightly higher compared to real-time PCR. Probit analysis showed that even a few genomic copies could be detected with 95% confidence. The microarrays detected DNA from different pathogens mixed in different ratios and from spiked or naturally contaminated samples. The assays that were developed have a potential for application in surveillance and diagnostics.
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