ArticleBiomicrofluidics2012
A tapered channel microfluidic device for comprehensive cell adhesion analysis, using measurements of detachment kinetics and shear stress-dependent motion.
Article in Biomicrofluidics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Theoretical and Experimental Studies of the Dynamic Damage of Endothelial Cellular Networks Under Ultrasound Cavitation.Cellular and molecular bioengineering · 2025Article
- A Microfluidic Design for Quantitative Measurements of Shear Stress-Dependent Adhesion and Motion ofBiomimetics (Basel, Switzerland) · 2024Article
- Catch bonds in sickle cell disease: Shear-enhanced adhesion of red blood cells to laminin.Biophysical journal · 2023Article
- The aerotaxis ofFrontiers in cell and developmental biology · 2023Article
- Article
- Tissue factor potentiates adherence of breast cancer cells to human umbilical vein endothelial cells under static and flow conditions.Cell adhesion & migration · 2021Article
- Single-cell adhesion force kinetics of cell populations from combined label-free optical biosensor and robotic fluidic force microscopy.Scientific reports · 2020Article
- Understanding mechanobiology in cultured endothelium: A review of the orbital shaker method.Atherosclerosis · 2019Review
- Hydrodynamics in Cell Studies.Chemical reviews · 2018Review
- Modulating wall shear stress gradient via equilateral triangular channel forBiomicrofluidics · 2016Article
- Interplay between motility and cell-substratum adhesion in amoeboid cells.Biomicrofluidics · 2015Article
- A Review of Cell Adhesion Studies for Biomedical and Biological Applications.International journal of molecular sciences · 2015Review
- The construction of an interfacial valve-based microfluidic chip for thermotaxis evaluation of human sperm.Biomicrofluidics · 2014Article
- Life under flow: A novel microfluidic device for the assessment of anti-biofilm technologies.Biomicrofluidics · 2013Article
- Microfluidic platforms for mechanobiology.Lab on a chip · 2013Review
- Perspective: Flicking with flow: Can microfluidics revolutionize the cancer research?Biomicrofluidics · 2013Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a method for studying cellular adhesion by using a custom-designed microfluidic device with parallel non-connected tapered channels. The design enables investigation of cellular responses to a large range of shear stress (ratio of 25) with a single input flow-rate. For each shear stress, a large number of cells are analyzed (500-1500 cells), providing statistically relevant data within a single experiment. Besides adhesion strength measurements, the microsystem presented in this paper enables in-depth analysis of cell detachment kinetics by real-time videomicroscopy. It offers the possibility to analyze adhesion-associated processes, such as migration or cell shape change, within the same experiment. To show the versatility of our device, we examined quantitatively cell adhesion by analyzing kinetics, adhesive strength and migration behaviour or cell shape modifications of the unicellular model cell organism Dictyostelium discoideum at 21 °C and of the human breast cancer cell line MDA-MB-231 at 37 °C. For both cell types, we found that the threshold stresses, which are necessary to detach the cells, follow lognormal distributions, and that the detachment process follows first order kinetics. In addition, for particular conditions' cells are found to exhibit similar adhesion threshold stresses, but very different detachment kinetics, revealing the importance of dynamics analysis to fully describe cell adhesion. With its rapid implementation and potential for parallel sample processing, such microsystem offers a highly controllable platform for exploring cell adhesion characteristics in a large set of environmental conditions and cell types, and could have wide applications across cell biology, tissue engineering, and cell screening.
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Registered trials
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