ArticleThe Korean journal of parasitology2011
Microarray analysis of differentially expressed genes between cysts and trophozoites of Acanthamoeba castellanii.
Article in The Korean journal of parasitology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Microarray-based characterization of airway inflammation induced by environmental Acanthamoeba exposure versus the ovalbumin-alum model.Parasites, hosts and diseases · 2025Article
- Proteases of Acanthamoeba.Parasitology research · 2023Review
- mRNA Sequencing Reveals Upregulation of Glutathione S-Transferase Genes duringMicroorganisms · 2023Article
- Biological characteristics and pathogenicity ofFrontiers in microbiology · 2023Review
- Acanthamoeba castellanii exhibits intron retention during encystment.Parasitology research · 2022Article
- A Novel Cysteine Protease Inhibitor ofPathogens (Basel, Switzerland) · 2021Article
- The role of the Acanthamoeba castellanii Sir2-like protein in the growth and encystation of Acanthamoeba.Parasites & vectors · 2020Article
- Cytopathic Change and Inflammatory Response of Human Corneal Epithelial Cells Induced by Acanthamoeba castellanii Trophozoites and Cysts.The Korean journal of parasitology · 2019Article
- DNA Methylation of Gene Expression inThe Korean journal of parasitology · 2017Article
- Identification and Characterization of Protein Arginine Methyltransferase 1 inThe Korean journal of parasitology · 2017Article
- Essential Role for an M17 Leucine Aminopeptidase in Encystation of Acanthamoeba castellanii.PloS one · 2015Article
- Down-regulation of cellulose synthase inhibits the formation of endocysts in Acanthamoeba.The Korean journal of parasitology · 2014Article
- Cysteine protease inhibitor (AcStefin) is required for complete cyst formation of Acanthamoeba.Eukaryotic cell · 2013Article
- Short-cut pathway to synthesize cellulose of encysting Acanthamoeba.The Korean journal of parasitology · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acanthamoeba infection is difficult to treat because of the resistance property of Acanthamoeba cyst against the host immune system, diverse antibiotics, and therapeutic agents. To identify encystation mediating factors of Acanthamoeba, we compared the transcription profile between cysts and trophozoites using microarray analysis. The DNA chip was composed of 12,544 genes based on expressed sequence tag (EST) from an Acanthamoeba ESTs database (DB) constructed in our laboratory, genetic information of Acanthamoeba from TBest DB, and all of Acanthamoeba related genes registered in the NCBI. Microarray analysis indicated that 701 genes showed higher expression than 2 folds in cysts than in trophozoites, and 859 genes were less expressed in cysts than in trophozoites. The results of real-time PCR analysis of randomly selected 9 genes of which expression was increased during cyst formation were coincided well with the microarray results. Eukaryotic orthologous groups (KOG) analysis showed an increment in T article (signal transduction mechanisms) and O article (posttranslational modification, protein turnover, and chaperones) whereas significant decrement of C article (energy production and conversion) during cyst formation. Especially, cystein proteinases showed high expression changes (282 folds) with significant increases in real-time PCR, suggesting a pivotal role of this proteinase in the cyst formation of Acanthamoeba. The present study provides important clues for the identification and characterization of encystation mediating factors of Acanthamoeba.
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