ArticleProceedings of the National Academy of Sciences of the United States of America2012
An engineered innate immune defense protects grapevines from Pierce disease.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 78 citations in OpenAlex.
- From Genome Inspection to Precision Agrochemicals: A Structure-Based Antivirulence Roadmap for Sustainable Crop Protection againstJournal of agricultural and food chemistry · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Genetic and physiological mechanisms of grapevine resistance toFrontiers in plant science · 2026Review
- Host-derived chimeric peptides clear the causative bacteria and augment host innate immunity during infection: A case study of HLB in citrus and fire blight in apple.Frontiers in plant science · 2022Article
- Article
- New Technologies and Strategies for Grapevine Breeding Through Genetic Transformation.Frontiers in plant science · 2021Review
- Proteome Analysis of Walnut Bacterial Blight Disease.International journal of molecular sciences · 2020Article
- Screening and identification of BP100 peptide conjugates active against Xylella fastidiosa using a viability-qPCR method.BMC microbiology · 2020Article
- Article
- Non-Lethal Effects ofMicroorganisms · 2019Article
- Review
- Update of the Scientific Opinion on the risks to plant health posed byEFSA journal. European Food Safety Authority · 2019Article
- Trans-Graft Protection Against Pierce's Disease Mediated by Transgenic Grapevine Rootstocks.Frontiers in plant science · 2019Article
- Xylella fastidiosa: an examination of a re-emerging plant pathogen.Molecular plant pathology · 2018Article
- Pierce's Disease of Grapevines: A Review of Control Strategies and an Outline of an Epidemiological Model.Frontiers in microbiology · 2018Review
- Molecular Profiling of Pierce's Disease Outlines the Response Circuitry ofFrontiers in plant science · 2018Article
- Effects of transgenic expression of Brevibacterium linens methionine gamma lyase (MGL) on accumulation of Tylenchulus semipenetrans and key aminoacid contents in Carrizo citrange.Plant molecular biology · 2017Article
- Characterization of the Xylella fastidiosa PD1311 gene mutant and its suppression of Pierce's disease on grapevines.Molecular plant pathology · 2017Article
- The Secreted Protease PrtA Controls Cell Growth, Biofilm Formation and Pathogenicity in Xylella fastidiosa.Scientific reports · 2016Article
- The Type II Secreted Lipase/Esterase LesA is a Key Virulence Factor Required for Xylella fastidiosa Pathogenesis in Grapevines.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
16 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We postulated that a synergistic combination of two innate immune functions, pathogen surface recognition and lysis, in a protein chimera would lead to a robust class of engineered antimicrobial therapeutics for protection against pathogens. In support of our hypothesis, we have engineered such a chimera to protect against the gram-negative Xylella fastidiosa (Xf), which causes diseases in multiple plants of economic importance. Here we report the design and delivery of this chimera to target the Xf subspecies fastidiosa (Xff), which causes Pierce disease in grapevines and poses a great threat to the wine-growing regions of California. One domain of this chimera is an elastase that recognizes and cleaves MopB, a conserved outer membrane protein of Xff. The second domain is a lytic peptide, cecropin B, which targets conserved lipid moieties and creates pores in the Xff outer membrane. A flexible linker joins the recognition and lysis domains, thereby ensuring correct folding of the individual domains and synergistic combination of their functions. The chimera transgene is fused with an amino-terminal signal sequence to facilitate delivery of the chimera to the plant xylem, the site of Xff colonization. We demonstrate that the protein chimera expressed in the xylem is able to directly target Xff, suppress its growth, and significantly decrease the leaf scorching and xylem clogging commonly associated with Pierce disease in grapevines. We believe that similar strategies involving protein chimeras can be developed to protect against many diseases caused by human and plant pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.