ArticleProceedings of the National Academy of Sciences of the United States of America2012
Rapid, efficient functional characterization and recovery of HIV-specific human CD8+ T cells using microengraving.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
63 citing papers in PubMed, 108 citations in OpenAlex.
- A high-throughput modular microfluidic platform for versatile functional assays at single-cell level.Microsystems & nanoengineering · 2026Article
- AI-driven computational methods and benchmarking for T-cell antigen identification.Briefings in bioinformatics · 2026Review
- High-Throughput Single-Cell Analysis of Local Nascent Protein Deposition in 3D Microenvironments via Extracellular Protein Identification Cytometry (EPIC).Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Single-cell cloning and its approaches.Frontiers in cell and developmental biology · 2025Review
- Characterization of AI-enhanced magnetophoretic transistors operating in a tri-axial magnetic field for on-chip bioparticle sorting.Scientific reports · 2024Article
- Increased vesicular dynamics and nanoscale clustering of IL-2 after T cell activation.Biophysical journal · 2024Article
- Exploring the Potential of Nanotechnology in Pediatric Healthcare: Advances, Challenges, and Future Directions.Pharmaceutics · 2023Review
- Microraft arrays for serial-killer CD19 chimeric antigen receptor T cells and single cell isolation.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2023Article
- Future of Digital Assays to Resolve Clinical Heterogeneity of Single Extracellular Vesicles.ACS nano · 2022Review
- T cell antigen discovery.Nature methods · 2021Review
- Interfacing Biomaterials with Synthetic T Cell Immunity.Advanced healthcare materials · 2021Review
- Antigen discovery tools for adaptive immune receptor repertoire research.Current opinion in systems biology · 2020Article
- Deep Profiling of Cellular Heterogeneity by Emerging Single-Cell Proteomic Technologies.Proteomics · 2020Review
- Advances of Single-Cell Protein Analysis.Cells · 2020Review
- Evaluation of Single-Cell Cytokine Secretion and Cell-Cell Interactions with a Hierarchical Loading Microwell Chip.Cell reports · 2020Article
- Single-cell analysis targeting the proteome.Nature reviews. Chemistry · 2020Review
- How single-cell immunology is benefiting from microfluidic technologies.Microsystems & nanoengineering · 2020Review
- Ultrasonically-guided flow focusing generates precise emulsion droplets for high-throughput single cell analyses.Journal of bioscience and bioengineering · 2019Article
- Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Functional TCR T cell screening using single-cell droplet microfluidics.Lab on a chip · 2018Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
Abstract
The nature of certain clinical samples (tissue biopsies, fluids) or the subjects themselves (pediatric subjects, neonates) often constrain the number of cells available to evaluate the breadth of functional T-cell responses to infections or therapeutic interventions. The methods most commonly used to assess this functional diversity ex vivo and to recover specific cells to expand in vitro usually require more than 10(6) cells. Here we present a process to identify antigen-specific responses efficiently ex vivo from 10(4)-10(5) single cells from blood or mucosal tissues using dense arrays of subnanoliter wells. The approach combines on-chip imaging cytometry with a technique for capturing secreted proteins--called "microengraving"--to enumerate antigen-specific responses by single T cells in a manner comparable to conventional assays such as ELISpot and intracellular cytokine staining. Unlike those assays, however, the individual cells identified can be recovered readily by micromanipulation for further characterization in vitro. Applying this method to assess HIV-specific T-cell responses demonstrates that it is possible to establish clonal CD8(+) T-cell lines that represent the most abundant specificities present in circulation using 100- to 1,000-fold fewer cells than traditional approaches require and without extensive genotypic analysis a priori. This rapid (<24 h), efficient, and inexpensive process should improve the comparative study of human T-cell immunology across ages and anatomic compartments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.