ArticleMolecular and cellular biology2012
A balance between Tel1 and Rif2 activities regulates nucleolytic processing and elongation at telomeres.
Article in Molecular and cellular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 45 citations in OpenAlex.
- Rif2 interaction with Rad50 counteracts Tel1 functions in checkpoint signalling and DNA tethering by releasing Tel1 from MRX binding.Nucleic acids research · 2024Article
- Post-Transcriptional and Post-Translational Modifications in Telomerase Biogenesis and Recruitment to Telomeres.International journal of molecular sciences · 2023Review
- Novel insights into the mechanism of cell cycle kinases Mec1(ATR) and Tel1(ATM).Critical reviews in biochemistry and molecular biology · 2021Article
- Inhibition of MRN activity by a telomere protein motif.Nature communications · 2021Article
- Sae2 and Rif2 regulate MRX endonuclease activity at DNA double-strand breaks in opposite manners.Cell reports · 2021Article
- DNA binding modes influence Rap1 activity in the regulation of telomere length and MRX functions at DNA ends.Nucleic acids research · 2020Article
- The nuclear pore complex prevents sister chromatid recombination during replicative senescence.Nature communications · 2020Article
- Tel1/ATM Signaling to the Checkpoint Contributes to Replicative Senescence in the Absence of Telomerase.Genetics · 2019Article
- Towards the Mechanism of Yeast Telomere Dynamics.Trends in cell biology · 2019Review
- Processing of DNA Double-Strand Breaks by the MRX Complex in a Chromatin Context.Frontiers in molecular biosciences · 2019Review
- Cell cycle-dependent spatial segregation of telomerase from sites of DNA damage.The Journal of cell biology · 2017Article
- Rad6-Bre1-mediated H2B ubiquitination regulates telomere replication by promoting telomere-end resection.Nucleic acids research · 2017Article
- Insights into the Roles of Midazolam in Cancer Therapy.Evidence-based complementary and alternative medicine : eCAM · 2017Review
- The mre11 A470 alleles influence the hereditability and the segregation of telosomes in Saccharomyces cerevisiae.PloS one · 2017Article
- Functions and regulation of the MRX complex at DNA double-strand breaks.Microbial cell (Graz, Austria) · 2016Review
- Tel1 and Rif2 Regulate MRX Functions in End-Tethering and Repair of DNA Double-Strand Breaks.PLoS biology · 2016Article
- Hypothesis: Paralog Formation from Progenitor Proteins and Paralog Mutagenesis Spur the Rapid Evolution of Telomere Binding Proteins.Frontiers in genetics · 2016Article
- Editorial: The Evolving Telomeres.Frontiers in genetics · 2016Article
- Length-dependent processing of telomeres in the absence of telomerase.Nucleic acids research · 2014Article
- Telomere uncapping at the crossroad between cell cycle arrest and carcinogenesis.Molecular & cellular oncology · 2014Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Generation of G-strand overhangs at Saccharomyces cerevisiae yeast telomeres depends primarily on the MRX (Mre11-Rad50-Xrs2) complex, which is also necessary to maintain telomere length by recruiting the Tel1 kinase. MRX physically interacts with Rif2, which inhibits both resection and elongation of telomeres. We provide evidence that regulation of telomere processing and elongation relies on a balance between Tel1 and Rif2 activities. Tel1 regulates telomere nucleolytic processing by promoting MRX activity. In fact, the lack of Tel1 impairs MRX-dependent telomere resection, which is instead enhanced by the Tel1-hy909 mutant variant, which causes telomerase-dependent telomere overelongation. The Tel1-hy909 variant is more robustly associated than wild-type Tel1 to double-strand-break (DSB) ends carrying telomeric repeat sequences. Furthermore, it increases the persistence at a DSB adjacent to telomeric repeats of both MRX and Est1, which in turn likely account for the increased telomere resection and elongation in TEL1-hy909 cells. Strikingly, Rif2 is unable to negatively regulate processing and lengthening at TEL1-hy909 telomeres, indicating that the Tel1-hy909 variant overcomes the inhibitory activity exerted by Rif2 on MRX. Altogether, these findings highlight a primary role of Tel1 in overcoming Rif2-dependent negative regulation of MRX activity in telomere resection and elongation.
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