ArticleJournal of dental research2012
MMP activity in the hybrid layer detected with in situ zymography.
Article in Journal of dental research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers, 4 of them syntheses that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
70 citing papers in PubMed, 4 syntheses or guidelines pooled it, 217 citations in OpenAlex.
- Presence of host and bacterial-derived collagenolytic proteases in carious dentin: a systematic review of ex vivo studies.Frontiers in cellular and infection microbiology · 2023Pooled it
- Pooled it
- Effect of 2% Chlorhexidine Following Acid Etching on Microtensile Bond Strength of Resin Restorations: A Meta-Analysis.Medicina (Kaunas, Lithuania) · 2019Pooled it
- Mechanisms regulating the degradation of dentin matrices by endogenous dentin proteases and their role in dental adhesion. A review.American journal of dentistry · 2014Pooled it
- The Effects of Immediate Dentin Sealing on the Enzymatic Activity and Bond Strength of Hybrid Ceramic CAD/CAM Onlays: A Comparative Study of Two Universal Adhesives.Dentistry journal · 2026Article
- A novel dual-reactive primer enhances bond durability and builds chemical interlocking structures at the caries-affected dentin-biomaterial interface.Materials today. Bio · 2026Article
- Development of multifunctional dental adhesive with 4-formylphenyl acrylate and nano-silver.Clinical oral investigations · 2026Article
- Effect of magnetic MMP removal on long-term dentin collagen stability.European journal of oral sciences · 2026Article
- Bioactive luting cements on the adhesive interface of fiber post and root dentin: a comparative study of bond strength, water interaction, and enzymatic degradation.Journal of applied oral science : revista FOB · 2026Article
- Evaluation of bioactive glass primer on dentin bonding durability and interface properties.BMC oral health · 2025Article
- Transdentinal effects of flavonoid-based primers applied to caries-affected dentin on pulp cells.Clinical oral investigations · 2025Review
- Two-Year Bonding Performances of a Universal Adhesive After Dentin Pre-Treatment With Two-Step Silver-Containing Solutions.The journal of adhesive dentistry · 2025Article
- Protective role of ellagic acid in the adhesive interface after dental bleaching.Scientific reports · 2025Article
- Durable Dentin Bonding with Multifunctional Methacrylated Proanthocyanidins.Journal of dental research · 2025Article
- Effects of penetration enhancers on the performance of irrigants for root canal disinfection.Clinical oral investigations · 2025Article
- Preparation and characterization of biomineralizing dentin bonding primer based on bioactive amphiphilic raspberry-like nanoparticles.BMC oral health · 2025Article
- 12 Months Results of Bond Strength and Endogenous Enzymatic Activity of Radicular Dentin Obturated With Bioceramic Sealer.The journal of adhesive dentistry · 2025Article
- A dual action approach of B-type proanthocyanidins for silencing enzymatic activity and sealing natural caries-affected dentin-resin interfaces.Journal of dentistry · 2025Article
- Synergistic strategy of riboflavin and lipoids to bioengineer resin dentin hybrid layer.Scientific reports · 2025Article
- Effects of Grape Seed Extract-Modified Etchants on Collagenolytic Activity, Interface Formation, and Bonding Longevity of Adhesive-Dentin Interfaces.Materials (Basel, Switzerland) · 2025Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 7 institutions in 4 countries.
Funding
Abstract
Dentinal proteases are believed to play an important role in the degradation of hybrid layers (HL). This study investigated the HL gelatinolytic activity by in situ zymography and functional enzyme activity assay. The hypotheses were that HLs created by an etch-and-rinse adhesive exhibit active gelatinolytic activity, and MMP-2 and -9 activities in dentin increase during adhesive procedures. Etched-dentin specimens were bonded with Adper Scotchbond 1XT and restored with composite. Adhesive/dentin interface slices were placed on microscope slides, covered with fluorescein-conjugated gelatin, and observed with a multi-photon confocal microscope after 24 hrs. Human dentin powder aliquots were prepared and assigned to the following treatments: A, untreated; B, etched with 10% phosphoric acid; or C, etched with 10% phosphoric acid and mixed with Scotchbond 1XT. The MMP-2 and -9 activities of extracts of dentin powder were measured with functional enzyme assays. Intense and continuous enzyme activity was detected at the bottom of the HL, while that activity was more irregular in the upper HL. Both acid-etching and subsequent adhesive application significantly increased MMP-2 and -9 activities (p < 0.05). The results demonstrate, for the first time, intrinsic MMP activity in the HL, and intense activation of matrix-bound MMP activity with both etching and adhesive application.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.