ArticleInfection and immunity2012
Mannheimia haemolytica and its leukotoxin cause macrophage extracellular trap formation by bovine macrophages.
Article in Infection and immunity, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed, 145 citations in OpenAlex.
- Review
- Macrophage Extracellular Traps in Immunity and Cancer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Neutrophil extracellular traps in diabetic wound healing: mechanisms, pathological roles, and therapeutic implications.Burns & trauma · 2026Review
- Extracellular traps, an ancient defense mechanism described in hemocytes of the tick Rhipicephalus microplus.Parasites & vectors · 2025Article
- Aerobic exercise alleviates allergic airway inflammation by suppressingNon-coding RNA research · 2025Article
- Leukocyte immunoglobulin-like receptor B2 regulates atherosclerosis progression by modulating macrophage extracellular trap formation in foam macrophages through the PI3K-AKT signaling pathway.Journal of cell communication and signaling · 2025Article
- The Dual Role of Macrophage Extracellular Traps in Host Defense and Disease: Mechanisms and Therapeutic Implications.Biomolecules · 2025Review
- Pentatrichomonas hominis induces extracellular traps formation of macrophages via the TLR2/NADPH/PAD4 pathway.Parasites & vectors · 2025Article
- The dynamic roles of macrophages extracellular traps (METs) in immune regulation.Archives of pharmacal research · 2025Review
- Mastitis-related Staphylococcus aureus-derived extracellular vesicles induce a pro-inflammatory response in bovine monocyte-derived macrophages.Scientific reports · 2025Article
- Early responses of primary human and bovine monocytes, monocytic THP-1 cells and THP-1 cell-derived macrophages to vitalFrontiers in immunology · 2025Article
- Macrophage extracellular traps are induced by Mycoplasma bovis in bovine macrophages through NADPH oxidase/ROS-dependent manner and their antibacterial efficacy.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- The role of extracellular traps released by neutrophils, eosinophils, and macrophages in asthma.Respiratory research · 2024Review
- Emerging insights into macrophage extracellular traps in bacterial infections.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Review
- Circulating Nucleosomes as a Novel Biomarker for Sepsis: A Scoping Review.Biomedicines · 2024Article
- Immune Cells in the Spleen of Mice Mediate the Inflammatory Response Induced byAnimals : an open access journal from MDPI · 2024Article
- Monocyte/macrophage-mediated venous thrombus resolution.Frontiers in immunology · 2024Review
- Macrophage extracellular traps require peptidylarginine deiminase 2 and 4 and are a source of citrullinated antigens bound by rheumatoid arthritis autoantibodies.Frontiers in immunology · 2024Article
- Impact of endogenous and exogenous nitrogen species on macrophage extracellular trap (MET) formation by bone marrow-derived macrophages.Cell and tissue research · 2023Article
- An in vitro model for Extracellular DNA Traps (ETs)-rich Human Thrombus Analogs.Journal of neurointerventional surgery · 2023Article
22 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human and bovine neutrophils release neutrophil extracellular traps (NETs), which are protein-studded DNA matrices capable of extracellular trapping and killing of pathogens. Recently, we reported that bovine neutrophils release NETs in response to the important respiratory pathogen Mannheimia haemolytica and its leukotoxin (LKT). Here, we demonstrate macrophage extracellular trap (MET) formation by bovine monocyte-derived macrophages exposed to M. haemolytica or its LKT. Both native fully active LKT and noncytolytic pro-LKT (produced by an lktC mutant of M. haemolytica) stimulated MET formation. Confocal and scanning electron microscopy revealed a network of DNA fibrils with colocalized histones in extracellular traps released from bovine macrophages. Formation of METs required NADPH oxidase activity, as previously demonstrated for NET formation. METs formed in response to LKT trapped and killed a portion of the M. haemolytica cells. Bovine alveolar macrophages, but not peripheral blood monocytes, also formed METs in response to M. haemolytica cells. MET formation was not restricted to bovine macrophages. We also observed MET formation by the mouse macrophage cell line RAW 264.7 and by human THP-1 cell-derived macrophages, in response to Escherichia coli hemolysin. The latter is a member of the repeats-in-toxin (RTX) toxin family related to the M. haemolytica leukotoxin. This study demonstrates that macrophages, like neutrophils, can form extracellular traps in response to bacterial pathogens and their exotoxins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.