ArticleInfection and immunity2012
The Vibrio cholerae Pst2 phosphate transport system is upregulated in biofilms and contributes to biofilm-induced hyperinfectivity.
Article in Infection and immunity, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Phosphate Limitation Modulates Vibrio Cholerae Outer Membrane Vesicle Formation, Composition and Toxicity.Journal of extracellular biology · 2026Article
- Serrapeptase EliminatesMicroorganisms · 2025Article
- Molecular characterization of Vibrio species isolated from dairy and water samples.Scientific reports · 2023Article
- Whole Genome Sequencing and Pan-Genomic Analysis of Multidrug-ResistantMicroorganisms · 2023Article
- New Insights into Vibrio cholerae Biofilms from Molecular Biophysics to Microbial Ecology.Advances in experimental medicine and biology · 2023Article
- Mechanisms Underlying the Virulence Regulation ofMicroorganisms · 2022Article
- The Vibrio cholerae master regulator for the activation of biofilm biogenesis genes, VpsR, senses both cyclic di-GMP and phosphate.Nucleic acids research · 2022Article
- Decreased biofilm formation in Proteus mirabilis after short-term exposure to a simulated microgravity environment.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Upregulation of virulence genes promotesProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Anti-biofilm Properties of the Fecal Probiotic Lactobacilli AgainstFrontiers in cellular and infection microbiology · 2018Article
- Comparative genomic, proteomic and exoproteomic analyses of three Pseudomonas strains reveals novel insights into the phosphorus scavenging capabilities of soil bacteria.Environmental microbiology · 2016Article
- Vibrio cholerae Biofilms and Cholera Pathogenesis.PLoS neglected tropical diseases · 2016Review
- Regulation of Type IV Pili Contributes to Surface Behaviors of Historical and Epidemic Strains of Clostridium difficile.Journal of bacteriology · 2016Article
- Mycobacterium tuberculosis Phosphate Uptake System Component PstA2 Is Not Required for Gene Regulation or Virulence.PloS one · 2016Article
- The RNA Domain Vc1 Regulates Downstream Gene Expression in Response to Cyclic Diguanylate in Vibrio cholerae.PloS one · 2016Article
- Two nucleotide second messengers regulate the production of the Vibrio cholerae colonization factor GbpA.BMC microbiology · 2015Article
- A systematic analysis of the in vitro and in vivo functions of the HD-GYP domain proteins of Vibrio cholerae.BMC microbiology · 2014Article
- Identification of genes induced in Vibrio cholerae in a dynamic biofilm system.International journal of medical microbiology : IJMM · 2014Article
- Acute toxicity of arsenic to Aliivibrio fischeri (Microtox bioassay) as influenced by potential competitive-protective agents.Environmental science and pollution research international · 2014Article
- Mannitol and the mannitol-specific enzyme IIB subunit activate Vibrio cholerae biofilm formation.Applied and environmental microbiology · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
Abstract
Vibrio cholerae is the causative agent of the deadly diarrheal disease cholera. As part of its life cycle, V. cholerae persists in marine environments, where it forms surface-attached communities commonly described as biofilms. Evidence indicates that these biofilms constitute the infectious form of the pathogen during outbreaks. Previous work has shown that biofilm-derived V. cholerae cells, even when fully dispersed from the biofilm matrix, are vastly more infectious than planktonic (free-living) cells. Here, we sought to identify factors that contribute to biofilm-induced hyperinfectivity in V. cholerae, and we present evidence for one aspect of the molecular basis of this phenotype. We identified proteins upregulated during growth in biofilms and determined their contributions to the hyperinfectivity phenotype. We found that PstS2, the periplasmic component of the Pst2 phosphate uptake system, was enriched in biofilms. Another gene in the pst2 locus was transcriptionally upregulated in biofilms. Using the infant mouse model, we found that mutation of two pst2 components resulted in impaired colonization. Importantly, deletion of the Pst2 inner membrane complex caused a greater colonization defect after growth in a biofilm compared to shaking culture. Based on these data, we propose that V. cholerae cells in biofilms upregulate the Pst2 system and therefore gain an advantage upon entry into the host. Further characterization of factors contributing to biofilm-induced hyperinfectivity in V. cholerae will improve our understanding of the transmission of the bacteria from natural aquatic habitats to the human host.
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