ArticlePlant physiology2012
Expression of an entire bacterial operon in plants.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Pyrrolnitrin is integral for antimicrobial activity and phenazine biosynthesis of Pseudomonas chlororaphis strains.World journal of microbiology & biotechnology · 2025Article
- Plant biomacromolecule delivery methods in the 21st century.Frontiers in genome editing · 2022Review
- Regulation of Flowering Timing by ABA-NnSnRK1 Signaling Pathway in Lotus.International journal of molecular sciences · 2021Article
- Harnessed viruses in the age of metagenomics and synthetic biology: an update on infectious clone assembly and biotechnologies of plant viruses.Plant biotechnology journal · 2019Review
- Antivirals against animal viruses.Biochemical pharmacology · 2017Review
- Rewiring carotenoid biosynthesis in plants using a viral vector.Scientific reports · 2017Article
- New biotechnological tools to accelerate scab-resistance trait transfer to apple.Genetics and molecular biology · 2017Article
- Expression of the entire polyhydroxybutyrate operon ofJournal of biological engineering · 2017Article
- Only minimal regions of tomato yellow leaf curl virus (TYLCV) are required for replication, expression and movement.Archives of virology · 2014Article
- Brassica villosa, a system for studying non-glandular trichomes and genes in the Brassicas.Plant molecular biology · 2014Article
- Advanced genetic tools for plant biotechnology.Nature reviews. Genetics · 2013Review
- Strategies for metabolic pathway engineering with multiple transgenes.Plant molecular biology · 2013Review
- Recent progress in the metabolic engineering of alkaloids in plant systems.Current opinion in biotechnology · 2013Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multigene expression is required for metabolic engineering, i.e. coregulated expression of all genes in a metabolic pathway for the production of a desired secondary metabolite. To that end, several transgenic approaches have been attempted with limited success. Better success has been achieved by transforming plastids with operons. IL-60 is a platform of constructs driven from the geminivirus Tomato yellow leaf curl virus. We demonstrate that IL-60 enables nontransgenic expression of an entire bacterial operon in tomato (Solanum lycopersicum) plants without the need for plastid (or any other) transformation. Delivery to the plant is simple, and the rate of expressing plants is close to 100%, eliminating the need for selectable markers. Using this platform, we show the expression of an entire metabolic pathway in plants and delivery of the end product secondary metabolite (pyrrolnitrin). Expression of this unique secondary metabolite resulted in the appearance of a unique plant phenotype disease resistance. Pyrrolnitrin production was already evident 2 d after application of the operon to plants and persisted throughout the plant's life span. Expression of entire metabolic pathways in plants is potentially beneficial for plant improvement, disease resistance, and biotechnological advances, such as commercial production of desired metabolites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.