ArticleThe Plant cell2012
Altered fermentative metabolism in Chlamydomonas reinhardtii mutants lacking pyruvate formate lyase and both pyruvate formate lyase and alcohol dehydrogenase.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 63 citations in OpenAlex.
- Intron retention dominates alternative splicing reprogramming during anaerobic stress in Chlamydomonas reinhardtii.Planta · 2026Article
- Photosynthetic Electron Flows and Networks of Metabolite Trafficking to Sustain Metabolism in Photosynthetic Systems.Plants (Basel, Switzerland) · 2024Review
- Occurrence, Evolution and Specificities of Iron-Sulfur Proteins and Maturation Factors in Chloroplasts from Algae.International journal of molecular sciences · 2021Review
- A Genome-Scale Metabolic Model ofMetabolites · 2021Article
- Phylogenetic and functional diversity of aldehyde-alcohol dehydrogenases in microalgae.Plant molecular biology · 2021Article
- Article
- Concerted Up-regulation of Aldehyde/Alcohol Dehydrogenase (ADHE) and Starch inThe Journal of biological chemistry · 2017Article
- An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii.The Plant cell · 2016Article
- Identification of the Elusive Pyruvate Reductase of Chlamydomonas reinhardtii Chloroplasts.Plant & cell physiology · 2016Article
- Metabolite Profiling ofFrontiers in plant science · 2016Article
- The role of pyruvate hub enzymes in supplying carbon precursors for fatty acid synthesis in photosynthetic microalgae.Photosynthesis research · 2015Article
- Multiple regulatory mechanisms in the chloroplast of green algae: relation to hydrogen production.Photosynthesis research · 2015Article
- Relevance of nutrient media composition for hydrogen production in Chlamydomonas.Photosynthesis research · 2015Review
- Enhanced acetyl-CoA production is associated with increased triglyceride accumulation in the green alga Chlorella desiccata.Journal of experimental botany · 2015Article
- Characterization of unusual truncated hemoglobins of Chlamydomonas reinhardtii suggests specialized functions.Planta · 2015Article
- The involvement of hydrogen-producing and ATP-dependent NADPH-consuming pathways in setting the redox poise in the chloroplast of Chlamydomonas reinhardtii in anoxia.The Journal of biological chemistry · 2015Article
- Alternative acetate production pathways in Chlamydomonas reinhardtii during dark anoxia and the dominant role of chloroplasts in fermentative acetate production.The Plant cell · 2014Article
- Article
- Evolutionary and biotechnological implications of robust hydrogenase activity in halophilic strains of Tetraselmis.PloS one · 2014Article
- Systems-level analysis of nitrogen starvation-induced modifications of carbon metabolism in a Chlamydomonas reinhardtii starchless mutant.The Plant cell · 2013Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlamydomonas reinhardtii, a unicellular green alga, often experiences hypoxic/anoxic soil conditions that activate fermentation metabolism. We isolated three Chlamydomonas mutants disrupted for the pyruvate formate lyase (PFL1) gene; the encoded PFL1 protein catalyzes a major fermentative pathway in wild-type Chlamydomonas cells. When the pfl1 mutants were subjected to dark fermentative conditions, they displayed an increased flux of pyruvate to lactate, elevated pyruvate decarboxylation, ethanol accumulation, diminished pyruvate oxidation by pyruvate ferredoxin oxidoreductase, and lowered H(2) production. The pfl1-1 mutant also accumulated high intracellular levels of lactate, succinate, alanine, malate, and fumarate. To further probe the system, we generated a double mutant (pfl1-1 adh1) that is unable to synthesize both formate and ethanol. This strain, like the pfl1 mutants, secreted lactate, but it also exhibited a significant increase in the levels of extracellular glycerol, acetate, and intracellular reduced sugars and a decrease in dark, fermentative H(2) production. Whereas wild-type Chlamydomonas fermentation primarily produces formate and ethanol, the double mutant reroutes glycolytic carbon to lactate and glycerol. Although the metabolic adjustments observed in the mutants facilitate NADH reoxidation and sustained glycolysis under dark, anoxic conditions, the observed changes could not have been predicted given our current knowledge of the regulation of fermentation metabolism.
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